IF (Immunofluorescence)
(Immunofluorescence staining of fixed Daudi cells with anti-CD37 antibody IPO-24 (ZC37-24) (AAA72157) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of IPO-24 (ZC37-24) () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Rabbit anti-Human CD37 Monoclonal Antibody | anti-CD37 antibody
Anti-CD37 [IPO-24 (ZC37-24)]
Gene Names
CD37; GP52-40; TSPAN26
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified antibody. Protein A affinity purified
Synonyms
CD37, Antibody; Anti-CD37 [IPO-24 (ZC37-24)]; Tetraspanin-26; Leukocyte antigen CD37; tetraspanin; Tspan-26; Gp52-40; anti-CD37 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
IPO-24 (ZC37-24)
Specificity
This antibody recognizes CD37, which is a cell surface protein, present mostly on mature B cells and B cell malignancies.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD37 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
PMID
11215818
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of fixed Daudi cells with anti-CD37 antibody IPO-24 (ZC37-24) (AAA72157) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of IPO-24 (ZC37-24) () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed Daudi cells with anti-CD37 antibody IPO-24 (ZC37-24) (AAA72157) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of IPO-24 (ZC37-24) () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western Blot using anti-CD37 antibody IPO-24 (ZC37-24) (AAA72157) Daudi cell lysate(A), human spleen(B), and tonsil(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of IPO-24 (ZC37-24) () at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-CD37 antibody IPO-24 (ZC37-24) (AAA72157) Daudi cell lysate(A), human spleen(B), and tonsil(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of IPO-24 (ZC37-24) () at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Related Product Information for anti-CD37 antibody
Recombinant monoclonal antibody to CD37. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma IPO-24 (ZC37-24).
This antibody was raised by immunizing mice with spleen cells of a patient with hairy cell leukemia.
This antibody was raised by immunizing mice with spleen cells of a patient with hairy cell leukemia.
Product Categories/Family for anti-CD37 antibody
References
Moldenhauer CD37. J Biol Regul Homeost Agents. 2000 Oct-Dec;14(4):281-3.
Note on publication: This article describes the characterization of the IPO-24 antibody.
Note on publication: This article describes the characterization of the IPO-24 antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,563 Da
NCBI Official Full Name
leukocyte antigen CD37 isoform B
NCBI Official Synonym Full Names
CD37 molecule
NCBI Official Symbol
CD37
NCBI Official Synonym Symbols
GP52-40; TSPAN26
NCBI Protein Information
leukocyte antigen CD37
UniProt Protein Name
Leukocyte antigen CD37
UniProt Gene Name
CD37
UniProt Synonym Gene Names
TSPAN26; Tspan-26
UniProt Entry Name
CD37_HUMAN
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Product Notes
The CD37 cd37 (Catalog #AAA72157) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD37 [IPO-24 (ZC37-24)] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD37 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD37 cd37 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD37, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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