WB (Western Blot)
( Western Blot using anti-CD38 antibody AT13/5 Human spleen lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of AT13/5 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD38 is 34.3kDa, but this protein is glycosylated at several residues. successfully detected CD38 in human spleen lysate.)
CD38 recombinant antibody
Anti-CD38 [AT13/5]
Gene Names
CD38; ADPRC1; ADPRC 1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
CD38, Recombinant Antibody; Anti-CD38 [AT13/5]; ADP-ribosyl cyclase 1; cyclic ADP-ribose hydrolase; EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38; p45; NAD(+) nucleosidase; CD38 recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, Kappa
Clone Number
AT13/5
Specificity
Binds specifically to human CD38, an approximately 45 kDa type II transmembrane protein, expressed on essentially all pre-B lymphocytes, plasma cells, and thymocytes. Also present on activated T lymphocytes, natural killer (NK) lymphocytes, myeloblasts, and erythroblasts. Bimodally expressed during B cell development, modulating from high in immature cells to low in intermediate ones and back to high on mature B cells. This antibody competes with clone HB7 (Ab00128) (Ellis 1995) which has been shon to bind to an epitope between amino acids 273-285).
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
300
Applicable Applications for CD38 recombinant antibody
FCM/FACS (Flow Cytometry)
Immunogen
Namalwa cells emulsified in CFA.
Buffer Composition
PBS only with 0.02% Proclin 300.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
( Western Blot using anti-CD38 antibody AT13/5 Human spleen lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of AT13/5 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD38 is 34.3kDa, but this protein is glycosylated at several residues. successfully detected CD38 in human spleen lysate.)
WB (Western Blot)
( Western Blot using anti-CD38 antibody AT13/5 Human spleen lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of AT13/5 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD38 is 34.3kDa, but this protein is glycosylated at several residues. successfully detected CD38 in human spleen lysate.)
IF (Immunofluorescence)
( Immunofluorescence staining of fixed Daudi cells with anti-CD38 antibody AT13/5 Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of AT13/5 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
( Immunofluorescence staining of fixed Daudi cells with anti-CD38 antibody AT13/5 Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of AT13/5 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for CD38 recombinant antibody
Recombinant monoclonal antibody to CD38. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AT13/5.
Namalwa cells emulsified in CFA.
Namalwa cells emulsified in CFA.
Product Categories/Family for CD38 recombinant antibody
References
Ellis et al. Engineered anti-CD38 monoclonal antibdies for immunotherapy of multiple myeloma. Journal of Immunology PMID:7608568
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,788 Da
NCBI Official Full Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
NCBI Official Synonym Full Names
CD38 molecule
NCBI Official Symbol
CD38
NCBI Official Synonym Symbols
ADPRC1; ADPRC 1
NCBI Protein Information
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
UniProt Protein Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
UniProt Gene Name
CD38
UniProt Synonym Gene Names
ADPRC 1; cADPr hydrolase 1
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Product Notes
The CD38 cd38 (Catalog #AAA71987) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD38 [AT13/5] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD38 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD38 cd38 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD38, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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