Mouse anti-Human CD45/LCA Monoclonal Antibody | anti-CD45/LCA antibody

CD45/LCA (Leucocyte Marker)

Cat. Num. AAA215171

• Gene Names: PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD45/LCA, Antibody; CD45/LCA (Leucocyte Marker); B220; CD45R; GP180; Leukocyte common antigen (LCA); Loc; Ly-5; Lyt-4; Protein tyrosine phosphatase receptor type C (PTPRC); Receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; anti-CD45/LCA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa + IgG1, kappa
• Clone Number: PTPRC/1147 + PTPRC/1460
• Specificity: Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1306

• Applicable Applications for anti-CD45/LCA antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Positive Control: Ramos, U-698 or GA-10 cells. Tonsil.
• Immunogen: Human recombinant full-length CD45 protein
• Cellular Localization: Cell Surface and Cytoplasmic
• Hu-chromosome Location: 1q31.3
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

WB (Western Blot)

(Western Blot Analysis of human spleen tissue lysates using CD45 Mouse Monoclonal Antibody (PTPRC/1147 + PTPRC/1460).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Monoclonal Antibody (PTPRC/1147 + PTPRC/1460).)

References

1. Michie SA et. al. American Journal of Clinical Pathology, 1987, 88(4):457-62. 2. Gatter KC et. al. Lancet, 1985 Jun 8, 1(8441):1302-5.

NCBI and Uniprot Product Information

• NCBI GI #: 392307007
• NCBI GeneID: 5788
• NCBI Accession #: NP_002829.3
• NCBI GenBank Nucleotide #: NP_002829.3
• UniProt Accession #: P08575
• Molecular Weight: 180-220kDa
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase C isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase receptor type C
• NCBI Official Symbol: PTPRC
• NCBI Official Synonym Symbols: LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: PTPRC
• UniProt Synonym Gene Names: CD45; L-CA
• UniProt Entry Name: PTPRC_HUMAN