IHC (Immunohistochemisry)
(CD45/LCA Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
Goat CD45/LCA Polyclonal Antibody | anti-PTPRC antibody
CD45/LCA Goat anti-Human Polyclonal (C-Terminus) Antibody
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Mouse, Dog, Rat, Human, Monkey
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunoaffinity purified
Synonyms
CD45/LCA, Antibody; CD45/LCA Goat anti-Human Polyclonal (C-Terminus) Antibody; PathPlus CD45/LCA Antibody (C-Terminus); PTPRC; B220; CD45R; CD45; gp180; Leukocyte common antigen; L-CA; Leucocyte common antigen; LY5; T200; T200 glycoprotein; T200 leukocyte common antigen; CD45 antigen; LCA; anti-PTPRC antibody
Host
Goat
Reactivity
Mouse, Dog, Rat, Human, Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
Using Jurkat cell lysate detects a band 180-220 kDa by Western blot.
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 0.05% Sodium Azide, 20% Glycerol
Concentration
3mg/ml (varies by lot)
Applicable Applications for anti-PTPRC antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Target
Human CD45/LCA
Immunogen
Purified recombinant peptide derived from within residues 1260 aa to the C-Terminus of human CD45 produced in E Coli.
Conjugation
Unconjugated
Epitope
C-Terminus
Family
Protein Phosphatase
Subfamily
R1/R6
Preparation and Storage
Short term: store at 4 degree C. Long term: aliquot and store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistochemisry)
(CD45/LCA Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemisry)
(CD45/LCA Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(CD45/LCA Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(CD45/LCA Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(CD45/LCA Antibody-Western blot. Endogenous CD45 detected with CD45 antibody at 1:500 dilution. Lysate at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.)
WB (Western Blot)
(CD45/LCA Antibody-Western blot. Endogenous CD45 detected with CD45 antibody at 1:500 dilution. Lysate at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.)
Related Product Information for anti-PTPRC antibody
LCA antibody is an unconjugated goat polyclonal antibody to LCA (CD45)(C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.
CD45 (LCA, PTPRC) is a type 1 transmembrane protein tyrosine phosphatase that is a marker for leukocytes. It is expressed on almost all hematopoietic cells (B and T-lymphocytes, granulocytes, monocytes, mast cells, dendritic cells, and macrophages). It is positive in many hematolymphoid neoplasms and may assist in the classification of lymphomas and leukemias. It is negative in mature erythrocytes, platelets, plasma cells, and mature megakaryocytes. Defects in PTPRC are one cause of severe combined immunodeficiency syndrome characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.
CD45 (LCA, PTPRC) is a type 1 transmembrane protein tyrosine phosphatase that is a marker for leukocytes. It is expressed on almost all hematopoietic cells (B and T-lymphocytes, granulocytes, monocytes, mast cells, dendritic cells, and macrophages). It is positive in many hematolymphoid neoplasms and may assist in the classification of lymphomas and leukemias. It is negative in mature erythrocytes, platelets, plasma cells, and mature megakaryocytes. Defects in PTPRC are one cause of severe combined immunodeficiency syndrome characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.
References
Hermiston et al. Annual review of immunology 21.1 (2003): 107-137, DOI: 10.1146/annurev.immunol.21.120601.140946;Am J Clin Pathol 1998;110:797, PMID: 9844593
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
147,254 Da
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase C isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
PTPRC
NCBI Official Synonym Symbols
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
PTPRC
UniProt Synonym Gene Names
CD45; L-CA
UniProt Entry Name
PTPRC_HUMAN
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Product Notes
The PTPRC ptprc (Catalog #AAA162694) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The CD45/LCA Goat anti-Human Polyclonal (C-Terminus) Antibody reacts with Mouse, Dog, Rat, Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD45/LCA can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PTPRC ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45/LCA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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