IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lymphoma stained with CD45RO Monoclonal Antibody (SPM125).)
Mouse CD45RO Monoclonal Antibody | anti-PTPRC antibody
CD45RO (T-Cell Marker) Mouse Monoclonal Antibody
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human, Chimpanzee, Common Marmoset
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
CD45RO, Antibody; CD45RO (T-Cell Marker) Mouse Monoclonal Antibody; T200, GP180, LCA, Leukocyte Common Antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein; anti-PTPRC antibody
Host
Mouse
Reactivity
Human, Chimpanzee, Common Marmoset
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
SPM125
Specificity
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
1306
Applicable Applications for anti-PTPRC antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Cell Surface
Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
Hu-Chromosome Location
1q31.3
Positive Control
CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lymphoma stained with CD45RO Monoclonal Antibody (SPM125).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lymphoma stained with CD45RO Monoclonal Antibody (SPM125).)
References
Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.2. Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138.3. Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315.4. Yamada M, et al. 1996. Stroke 27:1155. 5. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
180-185kDa
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase C isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type C
NCBI Official Symbol
PTPRC
NCBI Official Synonym Symbols
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
PTPRC
UniProt Synonym Gene Names
CD45; L-CA
UniProt Entry Name
PTPRC_HUMAN
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Product Notes
The PTPRC ptprc (Catalog #AAA62640) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD45RO (T-Cell Marker) Mouse Monoclonal Antibody reacts with Human, Chimpanzee, Common Marmoset and may cross-react with other species as described in the data sheet. AAA Biotech's CD45RO can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PTPRC ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45RO, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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