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WB (Western Blot)
(Detection of human CD45RO by western blot. Samples: Whole cell lysate (15 and 50 ug) from Jurkat cells prepared using NETN lysis buffer. Antibody: mouse anti-CD45RO antibody [UCHL-1] (AAA213504 Lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes.)
Mouse anti-Human CD45RO Monoclonal Antibody | anti-CD45RO antibody
Mouse anti-CD45RO Monoclonal Antibody, Purified [UCHL-1]
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
CD45RO, Antibody; Mouse anti-CD45RO Monoclonal Antibody, Purified [UCHL-1]; L-CA; protein tyrosine phosphatase, receptor type, c polypeptide; T200 leukocyte common antigen; T200 glycoprotein; receptor-type tyrosine-protein phosphatase C; LY5; Leukocyte common antigen; GP180; CD45R; B220; CD45 antigen; LCA; CD45; CD antigen CD45; T200; Receptor-type tyrosine-protein phosphatase C; protein tyrosine phosphatase, receptor type C; anti-CD45RO antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
UCHL-1
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for anti-CD45RO antibody
mIF (Multipleximmunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Cultured T-cells from an IL-2-dependent T-cell line (CA1)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD45RO by western blot. Samples: Whole cell lysate (15 and 50 ug) from Jurkat cells prepared using NETN lysis buffer. Antibody: mouse anti-CD45RO antibody [UCHL-1] (AAA213504 Lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human CD45RO by western blot. Samples: Whole cell lysate (15 and 50 ug) from Jurkat cells prepared using NETN lysis buffer. Antibody: mouse anti-CD45RO antibody [UCHL-1] (AAA213504 Lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human CD45RO (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Mouse anti-CD45RO monoclonal antibody [UCHL-1] (AAA213504 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-Mouse IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human CD45RO (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Mouse anti-CD45RO monoclonal antibody [UCHL-1] (AAA213504 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-Mouse IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemisry)
(Detection of human CD45RO by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD45RO antibody [UCHL-1] (AAA213504 Lot 1) used at a dilution of 1:250. Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohistochemisry)
(Detection of human CD45RO by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD45RO antibody [UCHL-1] (AAA213504 Lot 1) used at a dilution of 1:250. Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB. Counterstain: Hematoxylin (blue).)
ICC (Immunocytochemistry)
(Detection of human CD45RO by immunocytochemistry. Sample: FFPE section of human SR (lymphoma) cell line. Antibody: mouse monoclonal anti-CD45RO antibody [UCHL-1] (AAA213504 Lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD45RO by immunocytochemistry. Sample: FFPE section of human SR (lymphoma) cell line. Antibody: mouse monoclonal anti-CD45RO antibody [UCHL-1] (AAA213504 Lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
FCM/FACS (Flow Cytometry)
(Detection of human p53 (shaded) in Jurkat cells by flow cytometry. Antibody: Mouse anti-CD45RO recombinant monoclonal [UCHL-1] (AAA213504) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human p53 (shaded) in Jurkat cells by flow cytometry. Antibody: Mouse anti-CD45RO recombinant monoclonal [UCHL-1] (AAA213504) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG .)
Related Product Information for anti-CD45RO antibody
CD45RO is also known as protein tyrosine phosphatase receptor type C (PTPRC). It is a protein tyrosine phosphatase required for T-cell activation through the antigen receptor. CD45RO acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45RO recruits and dephosphorylates SKAP1 and FYN. It also dephosphorylates LYN, and thereby modulates LYN activity (By similarity)[taken from UniProtKB (Entry: P08575)].
Product Categories/Family for anti-CD45RO antibody
NCBI and Uniprot Product Information
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Product Notes
The CD45RO ptprc (Catalog #AAA213504) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-CD45RO Monoclonal Antibody, Purified [UCHL-1] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD45RO can be used in a range of immunoassay formats including, but not limited to, mIF (Multipleximmunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD45RO ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45RO, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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