Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO:Azide Free)
Mouse anti-Human CD45RO Monoclonal Antibody | anti-CD45RO antibody
MOUSE ANTI HUMAN CD45RO:RPE
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD45RO, Antibody; MOUSE ANTI HUMAN CD45RO:RPE; anti-CD45RO antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
UCHL1
Specificity
CD45RO
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Sequence Length
1304
Applicable Applications for anti-CD45RO antibody
FCM/FACS (Flow Cytometry)
Specificity Note
This product recognises the low molecular weight isoform (180kD) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cell with memory phenotype. In peripheral blood UCHL1 stains 40-80% of lymphocytes, and all monocytes and granulocytes.
Immunogen
Human IL-2 dependent T cells
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preservative Stabilisers
0.09% - Sodium Azide
1% - Bovine Serum Albumin
1% - Bovine Serum Albumin
Reconstitution
Reconstitute with 1 ml distilled water
Preparation and Storage
Prior to reconstitution store at +4 degree C. Following reconstitution store at +4 degree C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Stability: 12 months from date of reconstitution.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Stability: 12 months from date of reconstitution.
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO:Azide Free)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO:Azide Free)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO)
References
1. Smith, S.H. et al. (1986) Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology 58: 63-70.
2. Norton, A.J. et al. (1986) Monoclonal antibody (UCHL1) that recognises normal and neoplastic T-cells in routinely fixed tissues. J. Clin. Pathol. 39: 399-405.
3. Beverley, P.C.L. et al. (1986) T-cell subsets and function. Progress in Immunology VI. Cinader, B., Miller, G.G., eds. Academic Press Orlando pp 941-948.
4. Beverley, P.C.L. (1987) Human Tcell subsets. Immunology Letters 14: 263-267.
5. Terry. L.A. et al. (1987) Phenotypic heterogeneity of the CD4+ and CD8+ subsets. Leucocyte Typing III. McMichael, A.J., Beverley, P.C.L. et al. eds. University Press. pp 225-227.
6. Akbar, A.N. et al. (1988) Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T-cells. J. Immunol. 140: 2171 - 2178
7. Terry, L.A. et al. (1988) The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45 Immunology 64: 331-336.
8. Beverley, P.C.L. et al. (1988) Phenotypic diversity of the CD45 antigen and its relation to function. Immunology, Suppl. 1: 3-5.
9. Merkenschlager, M. et al. (1988) Limited dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T-cell memory formation. Eur. J. Immunol. 18: 1653-1661.
2. Norton, A.J. et al. (1986) Monoclonal antibody (UCHL1) that recognises normal and neoplastic T-cells in routinely fixed tissues. J. Clin. Pathol. 39: 399-405.
3. Beverley, P.C.L. et al. (1986) T-cell subsets and function. Progress in Immunology VI. Cinader, B., Miller, G.G., eds. Academic Press Orlando pp 941-948.
4. Beverley, P.C.L. (1987) Human Tcell subsets. Immunology Letters 14: 263-267.
5. Terry. L.A. et al. (1987) Phenotypic heterogeneity of the CD4+ and CD8+ subsets. Leucocyte Typing III. McMichael, A.J., Beverley, P.C.L. et al. eds. University Press. pp 225-227.
6. Akbar, A.N. et al. (1988) Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T-cells. J. Immunol. 140: 2171 - 2178
7. Terry, L.A. et al. (1988) The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45 Immunology 64: 331-336.
8. Beverley, P.C.L. et al. (1988) Phenotypic diversity of the CD45 antigen and its relation to function. Immunology, Suppl. 1: 3-5.
9. Merkenschlager, M. et al. (1988) Limited dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T-cell memory formation. Eur. J. Immunol. 18: 1653-1661.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
147,254 Da
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase C isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
PTPRC
NCBI Official Synonym Symbols
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; OTTHUMP00000033813; OTTHUMP00000033816; OTTHUMP00000033817; OTTHUMP00000038574; leukocyte common antigen; leukocyte-common antigen; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
PTPRC
UniProt Synonym Gene Names
CD45
UniProt Entry Name
PTPRC_HUMAN
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Product Notes
The CD45RO ptprc (Catalog #AAA49506) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CD45RO:RPE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD45RO can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD45RO ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45RO, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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