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Application Data
(Figure 3. Flow cytometry analysis of antigen binding of anti-human CD47 mAb(AAA11712). (A) AAA11712 does not bind to CHO-S cells that do not express CD47.(B) A clear peak shift of AAA11712 was seen compared to the control when incubated with CD47-expressing 8226 cells, indicating strong binding of AAA11712 to CD47. Antibodies were incubated at 5 ug mL.)
Human anti-Human CD47 Monoclonal Antibody | anti-CD47 antibody
Anti-CD47 (magrolimab biosimilar) mAb
Gene Names
CD47; IAP; OA3; MER6
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Synonyms
CD47, Antibody; Anti-CD47 (magrolimab biosimilar) mAb; CD47; MER6; IAP; anti-CD47 antibody
Host
Human
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5
% – 8% trehalose is added as protectants before
lyophilization. Please see Certificate of Analysis
for specific instructions of reconstitution
Applicable Applications for anti-CD47 antibody
ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:5000-10,000
Flow Cytometry 1:100
Flow Cytometry 1:100
Conjugate
Unconjugated
Target
CD47
Reconstitution
1.- Before opening the tube cap. Centrifuge the sample tube at 5000 for 3-5 min at room temperature to ensure the lyophilized sample to settle down at the bottom of the tube.
2.- Dissolved lyophilized protein sample in sterile water based on the recommended volume__86.21_ul. 3.- After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times.
Note: Do not vortex or vigorously pipette sample.
2.- Dissolved lyophilized protein sample in sterile water based on the recommended volume__86.21_ul. 3.- After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times.
Note: Do not vortex or vigorously pipette sample.
Preparation and Storage
Store at -20 degree to -80 degree for 12 months in lyophilized form. After reconsituttion if not intended to use within a month, aliquot and store -80 degree. ( Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Application Data
(Figure 3. Flow cytometry analysis of antigen binding of anti-human CD47 mAb(AAA11712). (A) AAA11712 does not bind to CHO-S cells that do not express CD47.(B) A clear peak shift of AAA11712 was seen compared to the control when incubated with CD47-expressing 8226 cells, indicating strong binding of AAA11712 to CD47. Antibodies were incubated at 5 ug mL.)
Application Data
(Figure 3. Flow cytometry analysis of antigen binding of anti-human CD47 mAb(AAA11712). (A) AAA11712 does not bind to CHO-S cells that do not express CD47.(B) A clear peak shift of AAA11712 was seen compared to the control when incubated with CD47-expressing 8226 cells, indicating strong binding of AAA11712 to CD47. Antibodies were incubated at 5 ug mL.)
Application Data
(Figure 2. Jurkat cell line were surface stained with anti-CD47 (AAA11712) (Blue histogram) and isotype control (Red histogram) 1 ug/ml followed by Alexa 488-conjugated Goat anti-Human IgG secondary antibody.)
Application Data
(Figure 2. Jurkat cell line were surface stained with anti-CD47 (AAA11712) (Blue histogram) and isotype control (Red histogram) 1 ug/ml followed by Alexa 488-conjugated Goat anti-Human IgG secondary antibody.)
Application Data
(Figure 1. ELISA plate pre-coated by 1 ?g/ml (100 ?l/well) Human CD47, mFc-His tagged protein can bind Anti-CD47?magrolimab biosimilar?IgG1? AAA11712 in a linear range of 3.2-16.0 ng/ml.)
Application Data
(Figure 1. ELISA plate pre-coated by 1 ?g/ml (100 ?l/well) Human CD47, mFc-His tagged protein can bind Anti-CD47?magrolimab biosimilar?IgG1? AAA11712 in a linear range of 3.2-16.0 ng/ml.)
Application Data
(Figure 3. Flow cytometry analysis of antigen binding of anti-human CD47 mAb(AAA11712). (A) AAA11712 does not bind to CHO-S cells that do not express CD47.(B) A clear peak shift of AAA11712 was seen compared to the control when incubated with CD47-expressing 8226 cells, indicating strong binding of AAA11712 to CD47. Antibodies were incubated at 5 ug mL.)
Application Data
(Figure 3. Flow cytometry analysis of antigen binding of anti-human CD47 mAb(AAA11712). (A) AAA11712 does not bind to CHO-S cells that do not express CD47.(B) A clear peak shift of AAA11712 was seen compared to the control when incubated with CD47-expressing 8226 cells, indicating strong binding of AAA11712 to CD47. Antibodies were incubated at 5 ug mL.)
Application Data
(Figure 2. Jurkat cell line were surface stained with anti-CD47 (AAA11712) (Blue histogram) and isotype control (Red histogram) 1 ug/ml followed by Alexa 488-conjugated Goat anti-Human IgG secondary antibody.)
Application Data
(Figure 2. Jurkat cell line were surface stained with anti-CD47 (AAA11712) (Blue histogram) and isotype control (Red histogram) 1 ug/ml followed by Alexa 488-conjugated Goat anti-Human IgG secondary antibody.)
Application Data
(Figure 1. ELISA plate pre-coated by 1 ?g/ml (100 ?l/well) Human CD47, mFc-His tagged protein can bind Anti-CD47?magrolimab biosimilar?IgG1? AAA11712 in a linear range of 3.2-16.0 ng/ml.)
Application Data
(Figure 1. ELISA plate pre-coated by 1 ?g/ml (100 ?l/well) Human CD47, mFc-His tagged protein can bind Anti-CD47?magrolimab biosimilar?IgG1? AAA11712 in a linear range of 3.2-16.0 ng/ml.)
Application Data
Application Data
Product Categories/Family for anti-CD47 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Leukocyte surface antigen CD47
NCBI Official Synonym Full Names
CD47 molecule
NCBI Official Symbol
CD47
NCBI Official Synonym Symbols
IAP; OA3; MER6
NCBI Protein Information
leukocyte surface antigen CD47; CD47 glycoprotein; Rh-related antigen; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; antigenic surface determinant protein OA3; antigen identified by monoclonal antibody 1D
UniProt Protein Name
Leukocyte surface antigen CD47
UniProt Gene Name
CD47
UniProt Synonym Gene Names
MER6; IAP
UniProt Entry Name
CD47_HUMAN
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Product Notes
The CD47 cd47 (Catalog #AAA11712) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD47 (magrolimab biosimilar) mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD47 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:5000-10,000 Flow Cytometry 1:100. Researchers should empirically determine the suitability of the CD47 cd47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD47, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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