Mouse anti-Human CD5 Monoclonal Antibody | anti-CD5 antibody

Anti-Hu CD5 Purified

Cat. Num. AAA128651

• Gene Names: CD5; T1; LEU1
• Reactivity: Human
• Applications: Immunocytochemistry, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: CD5, Antibody; Anti-Hu CD5 Purified; CD5 molecule; T1; LEU1; anti-CD5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a kappa
• Clone Number: L17F12
• Specificity: The mouse monoclonal antibody L17F12 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 495

• Applicable Applications for anti-CD5 antibody: ICC (Immunocytochemistry), WB (Western Blot), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry)
• Immunogen: Human acute lymphoblastic leukemia (ALL) T cells
• Preparation and Storage: Store at 2-8 degree C.

IHC (Immunohiostchemistry)

(Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD5 (L17F12), 10 ug/ml. Commercially tested by LifeSpan BioSciences.)

Related Product Information for anti-CD5 antibody

CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.

NCBI and Uniprot Product Information

• NCBI GI #: 166197668
• NCBI GeneID: 921
• NCBI Accession #: NP_055022.2
• NCBI GenBank Nucleotide #: NP_055022.2
• UniProt Accession #: P06127
• NCBI Official Full Name: T-cell surface glycoprotein CD5 isoform 1
• NCBI Official Synonym Full Names: CD5 molecule
• NCBI Official Symbol: CD5
• NCBI Official Synonym Symbols: T1; LEU1
• NCBI Protein Information: T-cell surface glycoprotein CD5
• UniProt Protein Name: T-cell surface glycoprotein CD5
• UniProt Gene Name: CD5
• UniProt Synonym Gene Names: LEU1
• UniProt Entry Name: CD5_HUMAN