ICC (Immunocytochemistry)
(Immunocytochemical labeling of CD63 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal CD63 (CM0401). The antibody was detected using goat anti-mouse Ig DyLight 594.)
Mouse anti-Human CD63 Monoclonal Antibody | anti-CD63 antibody
CD63 (Extracellular region) M040 Antibody
Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Purity
Protein G chromatography
Synonyms
CD63, Antibody; CD63 (Extracellular region) M040 Antibody; CD63 (Extracellular region); CD063 M040; anti-CD63 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
M040
Specificity
Clone M040 mouse monoclonal antibody has high reactivity to CD63 at the plasma membrane in immunocytochemical assays, and weakly detects a 30-60kDa* protein on SDS-PAGE "Native" immunoblots of human A431, MeWo, and A549 cells. The antibody does not detect denatured CD63 in western blot assays.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-CD63 antibody
WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Clone (M040) was generated from a proprietary antigen related to the native human CD63 expressed in MeWo melanoma cell line.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of CD63 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal CD63 (CM0401). The antibody was detected using goat anti-mouse Ig DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of CD63 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal CD63 (CM0401). The antibody was detected using goat anti-mouse Ig DyLight 594.)
WB (Western Blot)
(Western blot of native human cell lyastes: MeWo (lane 1), and MeWo treated with PNGase F (lane 2), and A549 (lane 3), and A549 treated with PNGase F (lane 4). The blot was probed with mouse monoclonal CD63 (CM0401) at 1:100.)
WB (Western Blot)
(Western blot of native human cell lyastes: MeWo (lane 1), and MeWo treated with PNGase F (lane 2), and A549 (lane 3), and A549 treated with PNGase F (lane 4). The blot was probed with mouse monoclonal CD63 (CM0401) at 1:100.)
Related Product Information for anti-CD63 antibody
Tetraspanins comprise a large superfamily of cell surface-associated membrane proteins with four transmembrane domains. On cell membranes, tetraspanins form networks of various proteins called tetraspanin-enriched microdomains (TEMs). CD63 was the first characterized tetraspanin and it is found in TEMs, as well as late endosomes and lysosomes. In late endosomes, CD63 is enriched on the intraluminal vesicles, and can be secreted as exosomes through fusion of endosomes with the plasma membrane. The complex localization pattern of CD63 suggests that its intracellular trafficking and distribution must be tightly regulated. CD63 contains N-link glycosylation sites that produce diverse CD63 molecules that range from 30 to 60kDa depending on cell type. CD63 is an important exosomal marker in cancer cells, and may be involved in cancer progression and metastasis.
References
Pols, MS & Klumperman, J (2009) Exp Cell Res. 315(9):1584.
Metzelaar, M.J. et al. (1991) J Biol Chem. 266(5):3239.
Azorsa, D.O. et al. (1991) Blood. 78(2):280.
Metzelaar, M.J. et al. (1991) J Biol Chem. 266(5):3239.
Azorsa, D.O. et al. (1991) Blood. 78(2):280.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,430 Da
NCBI Official Full Name
melanoma-associated antigen
NCBI Official Synonym Full Names
CD63 molecule
NCBI Official Symbol
CD63
NCBI Official Synonym Symbols
MLA1; ME491; LAMP-3; OMA81H; TSPAN30
NCBI Protein Information
CD63 antigen
UniProt Protein Name
CD63 antigen
UniProt Gene Name
CD63
UniProt Synonym Gene Names
MLA1; TSPAN30; LAMP-3; Tspan-30
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Product Notes
The CD63 cd63 (Catalog #AAA71587) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD63 (Extracellular region) M040 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD63 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the CD63 cd63 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD63, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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