WB (Western Blot)
(Detection of human CD69 by western blot. Samples: Whole cell lysate (10 ug) from MUTZ-3, Jurkat, HEK293T, MOLT-4, and U-937 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD69 recombinant monoclonal antibody (AAA213624 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
CD69 recombinant antibody
Rabbit anti-CD69 Recombinant Monoclonal Antibody [BLR170J]
Gene Names
CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Western Blot
Purity
Purified
Synonyms
CD69, Recombinant Antibody; Rabbit anti-CD69 Recombinant Monoclonal Antibody [BLR170J]; EA1; MLR-3; leukocyte surface antigen Leu-23; Activation inducer molecule; GP32/28; early T-cell activation antigen p60; early lymphocyte activation antigen; early activation antigen CD69; C-type lectin domain family 2 member C; CLEC2C; CD69 antigen (p60, early T-cell activation antigen); CD antigen CD69; BL-AC/P26; activation inducer molecule (AIM/CD69); AIM; C-type lectin domain family 2, member C; Early activation antigen CD69; CD69 molecule; CD69 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR170J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for CD69 recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Residues 64-199 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD69 by western blot. Samples: Whole cell lysate (10 ug) from MUTZ-3, Jurkat, HEK293T, MOLT-4, and U-937 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD69 recombinant monoclonal antibody (AAA213624 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human CD69 by western blot. Samples: Whole cell lysate (10 ug) from MUTZ-3, Jurkat, HEK293T, MOLT-4, and U-937 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD69 recombinant monoclonal antibody (AAA213624 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
ICC (Immunocytochemistry)
(Detection of human CD69 in FFPE MUTZ-3 cells by immunocytochemistry. Antibody: Rabbit anti-CD69 recombinant monoclonal antibody (AAA213624 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD69 in FFPE MUTZ-3 cells by immunocytochemistry. Antibody: Rabbit anti-CD69 recombinant monoclonal antibody (AAA213624 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM/FACS (Flow Cytometry)
(Detection of human CD69 (shaded) in Mutz-3 cells by flow cytometry. Antibody: Rabbit anti-CD69 recombinant monoclonal (AAA213624 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human CD69 (shaded) in Mutz-3 cells by flow cytometry. Antibody: Rabbit anti-CD69 recombinant monoclonal (AAA213624 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for CD69 recombinant antibody
CD69 is a member of the calcium dependent lectin superfamily of type II transmembrane receptors. CD69 expression is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets [taken from NCBI Entrez Gene (Gene ID: 969)].
Product Categories/Family for CD69 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,559 Da
NCBI Official Full Name
early activation antigen CD69
NCBI Official Synonym Full Names
CD69 molecule
NCBI Official Symbol
CD69
NCBI Official Synonym Symbols
AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
NCBI Protein Information
early activation antigen CD69
UniProt Protein Name
Early activation antigen CD69
UniProt Gene Name
CD69
UniProt Synonym Gene Names
CLEC2C; AIM
UniProt Entry Name
CD69_HUMAN
Similar Products
Product Notes
The CD69 cd69 (Catalog #AAA213624) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD69 Recombinant Monoclonal Antibody [BLR170J] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD69 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD69 cd69 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD69, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.