IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin- embedded human-skin, antibody was diluted at 1:100.)
Rabbit CD73 Polyclonal Antibody | anti-CD73 antibody
CD73 Rabbit pAb
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human, Rat, Mouse
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
CD73, Antibody; CD73 Rabbit pAb; NT5E; NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; CD73; anti-CD73 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CD73 Polyclonal Antibody detects endogenous levels of CD73 protein
Purity/Purification
Affinity Purified
Form/Format
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
574
Applicable Applications for anti-CD73 antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human NT5E. AA range:431-480
Organism
Human, Mouse
Cellular Localization
Cell membrane ; Lipid-anchor, GPI-anchor
Tissue specificity
Leukocyte, Liver, Placenta, Skin,
Function
Catalytic activity:A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate.,cofactor: Zinc., Disease:There is a decrease in the activity of NT5 in B-cell chronic lymphocytic leukemia.,Function:Hydrolyzes extracellular nucleotides into membrane permeable nucleosides., similarity: Belongs to the 5'-nucleotidase family.,subunit:Homodimer; disulfide-linked.
Preparation and Storage
Store at -15 degree C to -25 degree C/1 year (Do not lower than -25 degree C)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin- embedded human-skin, antibody was diluted at 1:100.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin- embedded human-skin, antibody was diluted at 1:100.)
WB (Western Blot)
(Western Blot analysis of MCF7, L929, K562 cells using CD73 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.)
WB (Western Blot)
(Western Blot analysis of MCF7, L929, K562 cells using CD73 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
68kD (Observed)
NCBI Official Full Name
5'-nucleotidase
NCBI Official Synonym Full Names
5'-nucleotidase ecto
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
NCBI Protein Information
5'-nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE; 5'-NT
UniProt Entry Name
5NTD_HUMAN
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Product Notes
The CD73 nt5e (Catalog #AAA268568) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD73 Rabbit pAb reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD73 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD73 nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD73, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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