Mouse CD74 Monoclonal Antibody | anti-CD74 antibody

CD74 (B-Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62657

• Gene Names: CD74; II; DHLAG; HLADG; Ia-GAMMA
• Reactivity: Human, Baboon, Mouse.; Does not react with Rat
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: CD74, Antibody; CD74 (B-Cell Marker) Mouse Monoclonal Antibody; CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain; anti-CD74 antibody

• Host: Mouse
• Reactivity: Human, Baboon, Mouse.; Does not react with Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: LN-2
• Specificity: It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-CD74 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cell Surface and paranuclear globular
• Immunogen: SU-DHL-4 lymphoma cells
• Hu-Chromosome Location: 5q33.1
• Positive Control: Daudi or Raji Cells. Tonsil or lymph node.
• Preparation and Storage: Antibody without azide- store at -20C to -80°C. Antibody is stable for 24 months. Non hazardous. No MSDS required.

WB (Western Blot)

(Western blot analysis of Raji cell lysate using CD74 Mouse Monoclonal Antibody (LN-2).)

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of Human Raji cells using CD74 Mouse Monoclonal Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).)

IF (Immunofluorescence)

(Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Mouse Monoclonal Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red))

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Spleen stained with CD74 Mouse Monoclonal Antibody (LN-2).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Monoclonal Antibody (LN-2).)

References

Epstein AL et. al. J of Immunology 133: 1028-1036, 1984. Marder RJ et. al. Lab Invest 52: 497-504, 1985

NCBI and Uniprot Product Information

• NCBI GeneID: 972
• UniProt Accession #: P044233; P04233
• Molecular Weight: 33-41kDa
• NCBI Official Synonym Full Names: CD74 molecule
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain