Goat anti-Human CD74/CLIP Polyclonal Antibody | anti-CD74 antibody

CD74/CLIP Goat anti-Human Polyclonal (aa159-171) Antibody

Cat. Num. AAA163327

• Gene Names: CD74; II; DHLAG; HLADG; Ia-GAMMA
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CD74/CLIP, Antibody; CD74/CLIP Goat anti-Human Polyclonal (aa159-171) Antibody; IHC-plus CD74/CLIP Antibody (aa159-171); CD74; CD74 antigen; DHLAG; HLADG; HLA-DR-gamma; Ia-associated invariant chain; p33; MHC HLA-DR gamma chain; Ia-GAMMA; anti-CD74 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This antibody is expected to recognize isoform a (NP_001020330.1) and isoform b (NP_004346.1).
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-CD74 antibody: WB (Western Blot), ELISA (Peptide ELISA), IHC (Immunohistochemistry)
• Target: Human CD74/CLIP
• Immunogen: CD74/CLIP antibody was raised against Peptide with sequence C-KGSFPENLRHLKN, from the internal region of the protein sequence according to NP_001020330.1NP_004346.1.
• Epitope: aa159-171
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

IHC (Immunohistochemistry)

(CD74/CLIP Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry)

(CD74/CLIP Antibody-Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemisry)

(CD74/CLIP Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot)

(CD74/CLIP Antibody-Antibody (0.01ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot)

(CD74/CLIP Antibody-Goat anti-CD74 (aa159-171) Antibody (0.01ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)

NCBI and Uniprot Product Information

• NCBI GI #: 10835071
• NCBI GeneID: 972
• NCBI Accession #: NP_004346.1
• NCBI GenBank Nucleotide #: NM_004355
• UniProt Accession #: P04233; Q14597; Q29832; Q5U0J8; Q8SNA0; Q8WLP6; A8K7R1; B4DNE8; D3DQG3; D3DQG4
• NCBI Official Full Name: HLA class II histocompatibility antigen gamma chain isoform b
• NCBI Official Synonym Full Names: CD74 molecule, major histocompatibility complex, class II invariant chain
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histo
• UniProt Protein Name: HLA class II histocompatibility antigen gamma chain
• UniProt Gene Name: CD74
• UniProt Synonym Gene Names: DHLAG; Ii
• UniProt Entry Name: HG2A_HUMAN