Mouse CD74 Monoclonal Antibody | anti-CD74 antibody

CD74 (B-Cell Marker)

Cat. Num. AAA215359

• Gene Names: CD74; II; p33; DHLAG; HLADG; Ia-GAMMA
• Reactivity: Human, Baboon, Mouse; Does not react with Rat
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD74, Antibody; CD74 (B-Cell Marker); DHLAG; Gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; HLADR-gamma (HLADG); Ia antigen-associated invariant chain; la-gamma; Major histocompatibility complex class II invariant chain; MHC HLA-DR gamma chain; anti-CD74 antibody

• Host: Mouse
• Reactivity: Human, Baboon, Mouse; Does not react with Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: rCLIP/813
• Specificity: It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-CD74 antibody: IHC (Immunohistochemistry)
• Immunogen: Recombinant full-length Human CD74 protein
• Cellular Localization: Cell Surfaceand Paranuclear Globular
• Antibody Type: Recombinant Antibody
• Hu-chromosome Location: 5q33.1
• Positive Control: 293T, Daudi or Raji cells. Tonsil or Lymph node.
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified CD74 Recombinant Mouse Monoclonal Antibody (rCLIP/813). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Recombinant Mouse Monoclonal Antibody (rCLIP/813).)

References

Epstein AL et. al. J of Immunology 133: 1028-1036, 1984. | Marder RJ et. al. Lab Invest 52: 497-504, 1985.

NCBI and Uniprot Product Information

• NCBI GeneID: 972
• UniProt Accession #: P044233; P04233
• Molecular Weight: 33-41kDa
• NCBI Official Synonym Full Names: CD74 molecule
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; p33; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain