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Application Data
(Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. Low power)
Mouse CD8 BETA Monoclonal Antibody | anti-CD8 BETA antibody
MOUSE ANTI RAT CD8 BETA
Gene Names
Cd8b; Cd8b1
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
CD8 BETA; Monoclonal Antibody; MOUSE ANTI RAT CD8 BETA; anti-CD8 BETA antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
341
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1 mg/ml (varies by lot)
Sequence Length
208
Applicable Applications for anti-CD8 BETA antibody
Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200
Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Immunogen
Rat/mouse T cell hybrids expressing CD8
Fusion Partners
Fusion Partners
Target Species
Rat
Preparation and Storage
Store at 4 degree C for one month or at -20 degree C for longer. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. Low power)
Application Data
(Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. Low power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)
Application Data
(Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. High power)
Application Data
(Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. High power)
Application Data
(Rat splenocytes stained with Mouse anti Rat CD8 Beta:FITC)
Application Data
(Rat splenocytes stained with Mouse anti Rat CD8 Beta:FITC)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)
Application Data
(Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. Medium power)
Application Data
(Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. Medium power)
Related Product Information for anti-CD8 BETA antibody
Mouse anti Rat CD8 beta antibody, clone 341 recognizes the beta chain of the rat CD8 cell surface antigen, expressed on thymocytes and peripheral cytotoxic/suppressor cells. CD8b is not expressed by NK cells or by intestinal intraepithelial T cells. Mouse anti Rat CD8 beta antibody, clone 341 is reported to block proliferative and cytotoxic responses of CD8 +ve effector cells in vitro.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,400 Da
NCBI Official Full Name
T-cell surface glycoprotein CD8 beta chain
NCBI Official Synonym Full Names
CD8b molecule
NCBI Official Symbol
Cd8b
NCBI Official Synonym Symbols
Cd8b1
NCBI Protein Information
T-cell surface glycoprotein CD8 beta chain; CD8 antigen 37 kDa chain; CD8 antigen beta-chain; CD8 antigen, beta chain; CD8 antigen, beta-chain; OX-8 membrane antigen
UniProt Protein Name
T-cell surface glycoprotein CD8 beta chain
UniProt Gene Name
Cd8b
UniProt Synonym Gene Names
Cd8b1
UniProt Entry Name
CD8B_RAT
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Product Notes
The CD8 BETA cd8b (Catalog #AAA12017) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD8 BETA can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD8 BETA cd8b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 BETA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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