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Application Data
(SDS-PAGE analysis of SU-9D5 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified SU-9D5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified SU-9D5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Mouse anti-Human CEACAM16 Monoclonal Antibody | anti-CEACAM16 antibody
anti-human CEACAM16 monoclonal antibody
Gene Names
CEACAM16; CEAL2; DFNA4B
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
CEACAM16, Antibody; anti-human CEACAM16 monoclonal antibody; anti-CEACAM16 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
SU-9D5
Specificity
anti-human CEACAM16
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
425
Applicable Applications for anti-CEACAM16 antibody
IHC (Immunohistochemistry), ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human CEACAM16
Selection
based on recognition of the complete native protein expressed on transfected
mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(SDS-PAGE analysis of SU-9D5 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified SU-9D5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified SU-9D5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(SDS-PAGE analysis of SU-9D5 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified SU-9D5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified SU-9D5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.3: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, 4, 6, 8 19, or 20. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (data not shown). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified SU-9D5 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM16 transfected cells (red curve).)
Application Data
(Fig.3: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, 4, 6, 8 19, or 20. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (data not shown). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified SU-9D5 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM16 transfected cells (red curve).)
Application Data
(Fig.2: Immunohistological staining using SU-9D5 Cat.# GM-0518 on paraffin sections of cochlea tissue of an adult transgenic mouse with a human CEACAM16 gene (Ceacam16+/+ mouse cochlea). Binding of biotinylated SU-9D5 was detected with horseradish peroxidase-coupled streptavidin and stained with 3-amino-9-ethylcarbazole. Constanze Schäuble, Tumor Immunology Laboratory, LMU, Munich)
Application Data
(Fig.2: Immunohistological staining using SU-9D5 Cat.# GM-0518 on paraffin sections of cochlea tissue of an adult transgenic mouse with a human CEACAM16 gene (Ceacam16+/+ mouse cochlea). Binding of biotinylated SU-9D5 was detected with horseradish peroxidase-coupled streptavidin and stained with 3-amino-9-ethylcarbazole. Constanze Schäuble, Tumor Immunology Laboratory, LMU, Munich)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using SU-9D5 Cat.# GM-0518. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM16 (red curve) or an irrelevant protein (control transfectant). Binding of SU-9D5 was detected with a PE- conjugated secondary antibody. A positive signal was obtained only with CEACAM16 transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using SU-9D5 Cat.# GM-0518. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM16 (red curve) or an irrelevant protein (control transfectant). Binding of SU-9D5 was detected with a PE- conjugated secondary antibody. A positive signal was obtained only with CEACAM16 transfected cells.)
Related Product Information for anti-CEACAM16 antibody
Background: CEA-related cell adhesion molecule 16 (CEACAM16) belongs to the carcinoembryonic antigen (CEA) gene family which are widely expressed (1). The mammal-specific member CEACAM16 is well conserved and specifically expressed in the inner ear (2). It is a structural component of the tectorial membrane and the expression is necessary for hearing over an extended frequency range (3).
References
Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin. Cancer Biol. 9, pp. 67-81.
Zimmermann W (2002). Carcinoembryonic antigen. In Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York, USA, pp. 459-462.
Kammerer R, Rüttiger L, Riesenberg R, Schäuble C, Krupar R, Kamp A, Sunami K, Eisenried A, Hennenberg M, Grunert F, Bress A, Battaglia S, Schrewe H, Knipper M, Schneider MR, Zimmermann W (2012). Loss of mammalspecific tectorial membrane component carcinoembryonic antigen cell adhesion molecule 16 (CEACAM16) leads to hearing impairment at low and high frequencies. Journal of biologicay chemistry 287,26, pp. 21584-21598
Zimmermann W (2002). Carcinoembryonic antigen. In Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York, USA, pp. 459-462.
Kammerer R, Rüttiger L, Riesenberg R, Schäuble C, Krupar R, Kamp A, Sunami K, Eisenried A, Hennenberg M, Grunert F, Bress A, Battaglia S, Schrewe H, Knipper M, Schneider MR, Zimmermann W (2012). Loss of mammalspecific tectorial membrane component carcinoembryonic antigen cell adhesion molecule 16 (CEACAM16) leads to hearing impairment at low and high frequencies. Journal of biologicay chemistry 287,26, pp. 21584-21598
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,873 Da
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 16
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 16
NCBI Official Symbol
CEACAM16
NCBI Official Synonym Symbols
CEAL2; DFNA4B
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 16; carcinoembryonic antigen like-2 protein
UniProt Gene Name
CEACAM16
UniProt Synonym Gene Names
CEAL2
UniProt Entry Name
CEA16_HUMAN
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Product Notes
The CEACAM16 ceacam16 (Catalog #AAA71480) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CEACAM16 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM16 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CEACAM16 ceacam16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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