IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
CHRM3 recombinant antibody
CHRM3 Antibody
Gene Names
CHRM3; HM3; EGBRS
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Affinity-chromatography
Synonyms
CHRM3, Recombinant Antibody; CHRM3 Antibody; Muscarinic acetylcholine receptor M3; CHRM3; CHRM3 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
2D4
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for CHRM3 recombinant antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antibody Type
Recombinant Antibody
Conjugation
Non-conjugated
Immunogen
A synthesized peptide derived from human Muscarinic Acetylcholine Receptor M3
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot)
(Western BlotPositive WB detected in:PC3 whole cell lysateAll lanes: Muscarinic Acetylcholine Receptor M3 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 67 kDaObserved band size: 67 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in:PC3 whole cell lysateAll lanes: Muscarinic Acetylcholine Receptor M3 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 67 kDaObserved band size: 67 kDa)
Related Product Information for CHRM3 recombinant antibody
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Product Categories/Family for CHRM3 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,128 Da
NCBI Official Full Name
muscarinic acetylcholine receptor M3
NCBI Official Synonym Full Names
cholinergic receptor, muscarinic 3
NCBI Official Symbol
CHRM3
NCBI Official Synonym Symbols
HM3; EGBRS
NCBI Protein Information
muscarinic acetylcholine receptor M3; acetylcholine receptor, muscarinic 3; m3 muscarinic receptor
UniProt Protein Name
Muscarinic acetylcholine receptor M3
UniProt Gene Name
CHRM3
UniProt Entry Name
ACM3_HUMAN
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Product Notes
The CHRM3 chrm3 (Catalog #AAA243963) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHRM3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHRM3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CHRM3 chrm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHRM3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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