Mouse Collagen Type IV Monoclonal Antibody

Mouse anti Collagen Type IV

Cat. Num. AAA77484

• Reactivity: Human; Does not react with other species
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: Collagen Type IV, Antibody; Mouse anti Collagen Type IV; anti-Collagen Type IV antibody

• Host: Mouse
• Reactivity: Human; Does not react with other species
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1043
• Specificity: 1043 recognizes type IV collagen in the extracellular - basement membranes of normal and pathological human tissues.
• Form/Format: Each vial contains 100 ul 1mg/ml of purified monoclonal antibody in PBS containing 0.09% sodium azide

• Applicable Applications for anti-Collagen Type IV antibody: WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
• Source Note: 1043 is a Mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with purified Human type IV collagen. The immunogen has been isolated from Human placenta.
• Preparation and Storage: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Application Data

(Figure 1. Strong collagen type IV specific staining of AAA77484 Clone 1043) in the basement membrane surrounding epithelial ducts and glomeruli in a frozen section of human kidney up to a 1:200 dilution. Also staining in the connective tissue and muscular layer of blood vessels. No staining of epithelial cells.)

Related Product Information for anti-Collagen Type IV antibody

The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasma. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiSerum is important in the differential identification of bullous lesions in dermatopathology.

Product Categories/Family for anti-Collagen Type IV antibody

Cancer

References

Visser, R., van der Beek, J.M.H., Havenith, M.G., Cleutjens, J.P.M. and Bosman, F.T. (1985). Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia. Histopathology 10, 171-180. Cleutjens, J.P.M., Havenith, M.G., Kasper, M., Vallinga, M., and Bosman, F.T. (1990). Absence of type IV collagen in the centre of the corneal epithelial basement membrane. Histochemical Journal 22, 688-94. Havenith, M.G. et al. (1987). Human specific anti-type IV collagen monoclonal antibodies, characterisation and immunohistochemical appliCation. Histochemistry 87, 123-28. Schapers, R.F.M., Pauwels, R.P.E., Havenith, M.G., Smeets, A.W.G.B, van den Brandt and P.A. Bosman, F.T. (1990). Prognostic Significance of type IV collagen and laminin immunoreactivity in urothelial carcinomas of the bladder. Cancer 66, 2583-88.