Mouse Collagen Type IV Monoclonal Antibody

Collagen Type IV

Cat. Num. AAA59203

• Reactivity: Human, Rat, Canine, Horse
• Synonyms: Collagen Type IV, Antibody; Collagen Type IV; anti-Collagen Type IV antibody

• Host: Mouse
• Reactivity: Human, Rat, Canine, Horse
• Clonality: Monoclonal
• Isotype: IgG1/K
• Clone Number: CIV22
• Form/Format: Anti-Collagen Type IV is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Control: Muscle, Lung
• Localization: Cytoplasmic
• Reactivity Note: Paraffin, Frozen
• Precautions: 1. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.; 2. Always wear personal protective equipment such as a laboratory coat, goggles, and gloves when handling reagents.; 3. Dispose of unused solution with copious amounts of water.; 4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 7. For additional safety information refer to the Safety Data Sheet for thisproduct.; 8. For complete recommendations for handling biological specimens, please referto the CDC document,
• Mounting Protocol IHC: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent-based resin such as PermaMounter.
• Mounting Protocol IF: 1. Bring FluoroMounter or FluoroMounter with DAPI to room temperature.; 2. Rinse slides with distilled or deionized water.; 3. Remove excess water from slides before laying them flat in the dark.; 4. Turn the media bottle upside down before opening the dropper bottle.; 5. Apply 1-3 drops of FluoroMounter to each slide making sure the specimen is covered.; 6. Incubate 3-5 minutes at room temperature in the dark.; 7. Coverslip.; 8. Observe under a fluorescent microscope using the appropriate filters.; 9. The slides are recommended to be stored at 2-8 0C in the dark.
• Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized, and incubation time), optimal performance should be established through the use of positive and negative controls.
• Preparation for Frozen Tissues Procedure: 1. Embed the specimen in OCT inside the cryostat.; 2. Cut sections at 5 microns.; 3. Place the section on a positively charged glass slide.; 4. Air dry for 30-60 minutes.; 5. Fix in acetone 100% for 2-10 minutes.; 6. Air dry for another 10 minutes.
• Preparation and Storage: Store at 2 to 8 degree C in the dark.

Application Data

Application Data

IF (Immunofluorescence)

(Inset: IHC and IF of Collagen Type IV on a FFPE Skin Tissue)

IHC (Immunohiostchemistry)

Dilution Info

Related Product Information for anti-Collagen Type IV antibody

Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. Collagen IV is a major constituent of the basement membranes, along with laminins and enactins. It is composed of the alpha 1 IV chain and alpha 2 IV chain in a 2:1 ratio. It can form insoluble fibers with high tensile strength. Normal tissue stains with this antibody in a manner consistent with the sites of mesenchymal elements and epithelial basal laminae. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas. Collagen IV can also be useful in the classification of soft tissue tumors; Schwanomas, Leiomyomas, and their well-differentiated malignant counterparts usually immunoreact to this antibody. The vascular nature of neoplasms, Hemangiopericytoma, Angiosarcoma and Epithelioid Hemangioendothelioma can be observed with this antibody.