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COMPLEMENT FACTOR I Monoclonal Antibody | anti-CFI antibody
MOUSE ANTI HUMAN COMPLEMENT FACTOR I
Gene Names
CFI; FI; IF; KAF; AHUS3; ARMD13; C3BINA; C3b-INA
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry, ELISA, Western Blot, Functional Assay
Synonyms
COMPLEMENT FACTOR I, Antibody; MOUSE ANTI HUMAN COMPLEMENT FACTOR I; anti-CFI antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
052.11.3 (3R/8)
Specificity
Completment Factor I
Form/Format
Purified IgG - liquid
Concentration
Approx. Protein Concentrations: Current, batch-specific concentration 1.20 mg/ml (varies by lot)
Applicable Applications for anti-CFI antibody
Flow Cytometry (FC), Immunohistochemistry-Frozen (IHC-Fr), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA), Western Blotting (WB), Functional Assays
Application Notes
ELISA: 1/500 - 1/4000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Human
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
RRID
AB_844540
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Native human factor I from serum.
Histology Positive Control
Tissue Kidney from post streptoccal glomerulonephritis patients
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
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Hazard Indicator
Related Product Information for anti-CFI antibody
Mouse anti Human Complement Factor I antibody, clone 3R/8 recognizes complement factor I, a member of the peptidase S1 family present in the blood plasma. Factor I is an 88 kDa protein consisting of one polypeptide chain of 35.4 kDa and one of 27.6 kDa. Factor I is a serine protease which inactivates complement components C3b and C4b. C3b cleavage requires the presence of cofactors complement component (3b/4b) receptor 1 (CR1), membrane cofactor protein or factor H, while C4b cleavage requires complement component 4 binding protein or CR1. Factor I has no natural inhibitors.
Dysfunctional factor I can lead to uncontrolled activation of the alternative complement pathway causing in systemic depletion of C3 and lower levels of factors B and H, resulting in recurrent pyogenic infections. Defects in CFI are also associated with haemolytic uraemic syndrome.
Mouse anti Human Complement Factor I antibody, clone 3R/8 blocks function of complement factor I.
Removal of Sodium Azide is recommended prior to use in functional assays.
Dysfunctional factor I can lead to uncontrolled activation of the alternative complement pathway causing in systemic depletion of C3 and lower levels of factors B and H, resulting in recurrent pyogenic infections. Defects in CFI are also associated with haemolytic uraemic syndrome.
Mouse anti Human Complement Factor I antibody, clone 3R/8 blocks function of complement factor I.
Removal of Sodium Azide is recommended prior to use in functional assays.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
complement factor I preproprotein
NCBI Official Synonym Full Names
complement factor I
NCBI Official Symbol
CFI
NCBI Official Synonym Symbols
FI; IF; KAF; AHUS3; ARMD13; C3BINA; C3b-INA
NCBI Protein Information
complement factor I; C3B/C4B inactivator; C3b-inactivator; Konglutinogen-activating factor; complement component I; complement control protein factor I; complement factor I heavy chain; light chain of factor I
UniProt Protein Name
Complement factor I
UniProt Gene Name
CFI
UniProt Synonym Gene Names
IF
UniProt Entry Name
CFAI_HUMAN
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Product Notes
The CFI cfi (Catalog #AAA11964) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COMPLEMENT FACTOR I can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunohistochemistry-Frozen (IHC-Fr), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA), Western Blotting (WB), Functional Assays. ELISA: 1/500 - 1/4000 Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CFI cfi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COMPLEMENT FACTOR I, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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