Mouse CRISPR Cas9 Monoclonal Antibody | anti-CRISPR antibody

Anti-CRISPR Cas9 Antibody

Cat. Num. AAA72759

• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Protein G Purified Culture Supernatant
• Synonyms: CRISPR Cas9, Antibody; Anti-CRISPR Cas9 Antibody; anti-CRISPR antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6F7
• Specificity: Specific for the Staphylococcus aureus Cas9 protein
• Purity/Purification: Protein G Purified Culture Supernatant
• Form/Format: 100 ul in PBS + 5 mM NaN3

• Applicable Applications for anti-CRISPR antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot)
• Immunogen Species: Mouse
• Immunogen Information: Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.
• Antigen: Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.
• Quality Control Tests: Western blots performed on each lot.
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence of HEK293 cells transfected with Cas9 and labeled with Cas9 antibody.)

WB (Western Blot)

(Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-).)

Related Product Information for anti-CRISPR antibody

CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015).

References

Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186-91.