Rabbit CRISPR-Cas9 Monoclonal Antibody | anti-SPy_1046 antibody

CRISPR-Cas9 SP Antibody

Cat. Num. AAA312479

• Reactivity: Recombinant Cas9 Streptococcus pyogenes
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Affinity-chromatography
• Synonyms: CRISPR-Cas9, Antibody; CRISPR-Cas9 SP Antibody; Cas9 antibody; CRISPR-associated endonuclease Cas9/Csn1 antibody; CRISPR-Cas9/Csn1 antibody; csn1 antibody; SpyCas9 antibody; anti-SPy_1046 antibody

• Host: Rabbit
• Reactivity: Recombinant Cas9 Streptococcus pyogenes
• Clonality: Monoclonal
• Clone Number: JM11-55
• Purity/Purification: Affinity-chromatography
• Form/Format: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

• Applicable Applications for anti-SPy_1046 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: Recombinant fragment derived from Streptococcus pyogenes
• Preparation and Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

IF (Immunofluorescence)

(Immunofluorescent analysis of 293T cells transfected with CRISPR-SpCas9, using CRISPR-Cas9 SP Antibody)

WB (Western Blot)

(Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.)

WB (Western Blot)

(Western blot analysis of CRISPR-Cas9 SP on 293 and 293T cells lysates transfected with CRISPR-Cas9 using anti- CRISPR-Cas9 SP antibody at 1/500 dilution.)

Related Product Information for anti-SPy_1046 antibody

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Product Categories/Family for anti-SPy_1046 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 15675041
• NCBI GeneID: 901176
• NCBI Accession #: NP_269215.1
• NCBI GenBank Nucleotide #: NC_002737.2
• UniProt Accession #: Q99ZW2
• Molecular Weight: Calculated MW: 158 kDa
• NCBI Official Full Name: conserved hypothetical protein
• NCBI Official Symbol: SPy_1046
• NCBI Protein Information: hypothetical protein
• UniProt Protein Name: CRISPR-associated endonuclease Cas9/Csn1
• UniProt Gene Name: cas9
• UniProt Entry Name: CAS9_STRP1