DB (Dot Blot)
(Dot blot analysis using Mouse Anti-Crotonaldehyde Monoclonal Antibody, Clone 2A8.1. Primary Antibody: Mouse Anti-Crotonaldehyde Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.)
Mouse anti-ALL Crotonaldehyde Monoclonal Antibody | anti-CRA antibody
Crotonaldehyde Antibody, Clone 2A8.1: HRP
Reactivity
ALL
Applications
ELISA, Western Blot
Purity
Protein G Purified
Synonyms
Crotonaldehyde, Antibody; Crotonaldehyde Antibody, Clone 2A8.1: HRP; Crotonaldehyde Antibody, Croton aldehyde Antibody, Crotonaldehyde (CRA) Antibody, CRA Antibody, Crotonaldehyde conjugated protein antibody, Crotonaldehyde modified protein antibody, Crotonaldehyde-conjugated protein antibody, Crotonaldehyde-modified protein antibody, CRA-lysine adduct Antibody, Crotonaldehyde-lysine adduct Antibody ; anti-CRA antibody
Host
Mouse
Reactivity
ALL
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2A8.1
Specificity
Specific for Crotonaldehyde modified proteins. Does not cross-react with Acrolein, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-CRA antibody
ELISA, WB (Western Blot)
Immunogen
Crotonaldehyde modified Keyhole Limpet Hemocyanin (KLH)
Certificate of Analysis
A 1:1000 dilution of MBS809001 was sufficient for detection of Crotonaldehyde-modified proteins in 1 ug of Crotonaldehyde-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Preparation and Storage
Ships on Blue Ice. Store at -20 degree C for 1 year +. Avoid freeze/thaw cycle.
DB (Dot Blot)
(Dot blot analysis using Mouse Anti-Crotonaldehyde Monoclonal Antibody, Clone 2A8.1. Primary Antibody: Mouse Anti-Crotonaldehyde Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.)
DB (Dot Blot)
(Dot blot analysis using Mouse Anti-Crotonaldehyde Monoclonal Antibody, Clone 2A8.1. Primary Antibody: Mouse Anti-Crotonaldehyde Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.)
Related Product Information for anti-CRA antibody
Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Crotonaldehyde (CRA) is a carcinogenic, conjugated aldehyde produced endogenously via lipid peroxidation. It covalently modifies biomolecules, forming adducts with DNA and nucleophilic amino acid residues of proteins such as lysine (1).
Product Categories/Family for anti-CRA antibody
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Product Notes
The CRA (Catalog #AAA104165) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Crotonaldehyde Antibody, Clone 2A8.1: HRP reacts with ALL and may cross-react with other species as described in the data sheet. AAA Biotech's Crotonaldehyde can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CRA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Crotonaldehyde, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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