Rabbit NKX6-1 Polyclonal Antibody | anti-NKX6-1 antibody

NKX6-1 Antibody (C-term)

Cat. Num. AAA288814

• Gene Names: NKX6-1; NKX6A; NKX6.1
• Reactivity: Human, mouse (Predicted Reactivity: Rat)
• Applications: ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: NKX6-1, Antibody; NKX6-1 Antibody (C-term); Homeobox protein Nkx-61; Homeobox protein NK-6 homolog A; NKX6-1; NKX6A; anti-NKX6-1 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This NKX6-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-314 amino acids from the C-terminal region of human NKX6-1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 6-1
• Sequence Length: 367

• Applicable Applications for anti-NKX6-1 antibody: ELISA, WB (Western Blot)
• Function: Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (By similarity). Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity).
• Cellular Location: Nucleus.
• Tissue Location: Pancreatic beta cells.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(NKX6-1 Antibody (C-term) (AAA288814) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NKX6-1 antibody detected the NKX6-1 protein (arrow).)

WB (Western Blot)

(NKX6-1 Antibody (C-term) (AAA288814) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NKX6-1 antibody detected the NKX6-1 protein (arrow).)

Related Product Information for anti-NKX6-1 antibody

In the pancreas, NKX6.1 is required for the development of
beta cells and is a potent bifunctional transcription regulator
that binds to AT-rich sequences within the promoter region of
target genes Iype et al. (2004) [PubMed 15056733].[supplied by
OMIM].

Product Categories/Family for anti-NKX6-1 antibody

Developmental Biology; Signal Transduction

References

Donelan, W., et al. J. Biol. Chem. 285(16):12181-12189(2010)
Zhu, S., et al. Dig. Dis. Sci. 54(5):996-1002(2009)
Schisler, J.C., et al. Mol. Cell. Biol. 28(10):3465-3476(2008)
Yokoi, N., et al. Diabetes 55(8):2379-2386(2006)
Hori, Y., et al. PLoS Med. 2 (4), E103 (2005) :

NCBI and Uniprot Product Information

• NCBI GI #: 111120318
• NCBI GeneID: 4825
• NCBI Accession #: NP_006159.2
• NCBI GenBank Nucleotide #: NM_006168.2
• UniProt Accession #: P78426
• Molecular Weight: Calculated MW: 37849
• NCBI Official Full Name: homeobox protein Nkx-6.1
• NCBI Official Synonym Full Names: NK6 homeobox 1
• NCBI Official Symbol: NKX6-1
• NCBI Official Synonym Symbols: NKX6A; NKX6.1
• NCBI Protein Information: homeobox protein Nkx-6.1
• UniProt Protein Name: Homeobox protein Nkx-6.1
• UniProt Gene Name: NKX6-1
• UniProt Synonym Gene Names: NKX6A
• UniProt Entry Name: NKX61_HUMAN