IHC (Immunohiostchemistry)
Cytokeratin 14 Antibody | anti-KRT14 antibody
Cytokeratin 14
Reactivity
Paraffin, Frozen
Synonyms
Cytokeratin 14; N/A; anti-KRT14 antibody
Host
Mouse Monoclonal
Reactivity
Paraffin, Frozen
Isotype
IgG3
Immunogen
Synthetic peptide of 15 amino acid residues from the C-terminus of the human cytokeratin 14.
Antibody Type
Mouse Monoclonal
Clone
LL002
Localization
Cytoplasmic
Species Reactivity
Human, Rat
Control
Squamous Mucosa, Prostate, Breast, Tonsil, Salivary Gland, Skin, Cervix Carcinoma, Squamous Carcinoma
Application
Breast Cancer,Melanoma & Skin Cancer, Kidney & Urothelial Cancer
Presentation
Anti-Cytokeratin 14 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5,containing BSA and sodium azide as a preservative.
Precautions
1. For professional users only. Results should be interpreted by a qualified medical professional.
2. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.
3. Always wear personal protective equipment such as a laboratorycoat, goggles, and gloves when handling reagents.
4. Dispose of unused solution with copious amounts of water.
5. Do not ingest reagent. If reagent is ingested,seek medical advice immediately.
6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
8. For additional safetyinformation refer to Safety Data Sheet for this product.
9. For complete recommendations for handling biological specimens, please refer to the CDC document,“Guidelines for Safe Work Practices in Human and AMDL” (see References in this document).
2. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.
3. Always wear personal protective equipment such as a laboratorycoat, goggles, and gloves when handling reagents.
4. Dispose of unused solution with copious amounts of water.
5. Do not ingest reagent. If reagent is ingested,seek medical advice immediately.
6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
8. For additional safetyinformation refer to Safety Data Sheet for this product.
9. For complete recommendations for handling biological specimens, please refer to the CDC document,“Guidelines for Safe Work Practices in Human and AMDL” (see References in this document).
Stability
This product is stable up to the expiration date on the product label.
Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use and avoid prolonged exposure to room temperature conditions.
Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use and avoid prolonged exposure to room temperature conditions.
Specimen Preparation
Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA, or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNAWasher solutions.
Frozen sections and cell preparations
The antibody can be used on acetone-fixed frozen sections and acetone-fixed cell preparations.
IHC Protocol
1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positivelycharged slides such as Hydrophilic Plus Slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate, and rehydrate tissues.
4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate.
5. Any of three heating methods may be used:
a. TintoRetriever Pressure Cooker or Equivalent Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.
c. Conventional Steamer Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual IHC, perform antibody incubation at ambient temperature. For automated IHC methods, perform antibody incubation according to instrument manufacturer’s instructions.
8. Wash slides with ImmunoDNA washer or DI water.
9. Continue IHC protocol. Wash slides between each step with ImmunoDNA washer solution.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate, and rehydrate tissues.
4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate.
5. Any of three heating methods may be used:
a. TintoRetriever Pressure Cooker or Equivalent Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.
c. Conventional Steamer Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual IHC, perform antibody incubation at ambient temperature. For automated IHC methods, perform antibody incubation according to instrument manufacturer’s instructions.
8. Wash slides with ImmunoDNA washer or DI water.
9. Continue IHC protocol. Wash slides between each step with ImmunoDNA washer solution.
Preparation and Storage
Store at 2-8°C (Control Slides: Store at 20-25°C)
IHC (Immunohiostchemistry)
IHC (Immunohiostchemistry)
IHC (Immunohistochemistry)
(IHC of Cytokeratin 14 on a FFPE Cervical Cancer Tissue)
IHC (Immunohistochemistry)
(IHC of Cytokeratin 14 on a FFPE Cervical Cancer Tissue)
Related Product Information for anti-KRT14 antibody
Cytokeratin 14 is a Type I polypeptide found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. It is usually found as a heterotetramer with two cytokeratin 5 molecules, and a Type II keratin. Together, they form the cytoskeleton of epithelial cells. Mutations in the genes for these cytokeratins are associated with Epidermolysis Bullosa Simplex. Cytokeratin 14 has been studied as a prognostic marker in Breast Cancer. This antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern is cytoplasmic. It recognizes Basal Cell Carcinomas and Squamous Cell Carcinomas. Anti-CK14 has been demonstrated to be useful in differentiating Squamous Cell Carcinomas from other epithelial tumors. This antibody has also been useful in separating oncocytic tumors of the kidney from renal mimics, as well as in determining metaplastic Carcinomas of the Breast.
This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections, and cell preparations. Interpretation of results should be performed by a qualified medical professional.
This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections, and cell preparations. Interpretation of results should be performed by a qualified medical professional.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Cytokeratin 14 krt14 (Catalog #AAA59259) is an Antibody produced from Mouse Monoclonal and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 14 reacts with Paraffin, Frozen and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "Cytokeratin 14, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.