Mouse anti-Human Cytokeratin 19 Monoclonal Antibody | anti-KRT19 antibody

Cytokeratin 19

Cat. Num. AAA59289

• Reactivity: Human
• Synonyms: Cytokeratin 19, Antibody; Cytokeratin 19; anti-KRT19 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1/K
• Clone Number: BSB-34

• Immunogen: A synthetic peptide against N-terminus of human cytokeratin 19 protein.
• Localization: Cytoplasmic
• Control: Colon, Bladder, Thyroid Carcinoma, Colon Carcinoma
• Precautions: 1. For professional users only. Results should be interpreted by a qualified medical professional.; 2. This product contains <0.1% sodium azide (NaN?) as a preservative. Ensure proper handling procedures are used with this reagent.; 3. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.; 4. Dispose of unused solution with copious amount of water.; 5. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 8. For additional safety information refer to Safety Data Sheet for this product.; 9. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL”.
• Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using Bio SB ImmunoDNA Retriever with Citrate (BSB 0020-BSB 0023), ImmunoDNA Retriever with EDTA (Inquire) or ImmunoDNA Digestor (Inquire). See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions (Inquire).; Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positively charged slides such as Hydrophilic Plus Slides (Inquire).; 2. Air dry for 2 hours at 58° C.; 3. Deparaffinize, dehydrate and rehydrate tissues.; 4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (Inquire) or EDTA (Inquire).; 5. Any of three heating methods may be used:; ; a. TintoRetriever Pressure Cooker or Equivalent; Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.; b. TintoRetriever PT Module or Water Bath Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.; c. Conventional Steamer Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.; ; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.; 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.; 8. Wash slides with ImmunoDNA washer or DI water.; 9. Continue IHC staining protocol. Wash slides between each step with ImmunoDNA washer solution.
• Mounting Protocols: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter contact Customer Service.
• Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls. Results should be interpreted by a qualified medical professional.
• Preparation and Storage: Store at 2-8°C (Control Slides: Store at 20-25°C)

Application Data

(Performance Characteristics)

IHC (Immunohiostchemistry)

(IHC of Cytokeratin 19 on an FFPE Thyroid Carcinoma Tissue)

Dilution Info

Related Product Information for anti-KRT19 antibody

Cytokeratin 19 is a Type I cytokeratin. Unlike its related family members, this smallest-known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically found in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Anti-Cytokeratin 19 reacts with a wide variety of epithelium and epithelial malignancies including Adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver and breast. Perhaps the most useful application is the identification of Thyroid Carcinoma of the papillary type, although Follicular Carcinoma is also labeled bythis antibody approximately 50-60% of the time. Cytokeratin 19 is not expressed in hepatocytes; therefore, this antibody is useful in the identification of liver metastasis. The degree of Cytokeratin 19 positivity in Breast Cancer distinguishes malignant from benign tumors. Cytokeratin 19 is often coexpressed with Cytokeratin 7.

NCBI and Uniprot Product Information

• NCBI GI #: 13537138
• NCBI Official Full Name: cytokeratin 19, partial
• UniProt Protein Name: Keratin, type I cytoskeletal 19
• UniProt Gene Name: KRT19
• UniProt Synonym Gene Names: CK-19; K19
• UniProt Entry Name: K1C19_HUMAN