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WB (Western Blot)
(Detection of human Cytokeratin-20 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, MOLT-4, HT-29, Hep-G2, HDLM-2, and MDA-MB-231 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Cytokeratin-20 recombinant monoclonal antibody (AAA213580 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)
Cytokeratin 20 Recombinant Antibody | KRT20 recombinant antibody
Rabbit anti-Cytokeratin 20 Recombinant Monoclonal Antibody [BLR105H]
Gene Names
KRT20; K20; CD20; CK20; CK-20; KRT21
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
Cytokeratin 20, Recombinant Antibody; Rabbit anti-Cytokeratin 20 Recombinant Monoclonal Antibody [BLR105H]; keratin 20, type I; KRT21; Keratin-20; CK-20; keratin, type I cytoskeletal 20; protein IT; K20; cytokeratin 20; CK20; CD20; Cytokeratin-20; Keratin, type I cytoskeletal 20; keratin 20; KRT20 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR105H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for KRT20 recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 250 and 300
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Cytokeratin-20 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, MOLT-4, HT-29, Hep-G2, HDLM-2, and MDA-MB-231 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Cytokeratin-20 recombinant monoclonal antibody (AAA213580 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human Cytokeratin-20 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, MOLT-4, HT-29, Hep-G2, HDLM-2, and MDA-MB-231 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Cytokeratin-20 recombinant monoclonal antibody (AAA213580 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human Cytokeratin-20 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HT-29 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Cytokeratin-20 recombinant monoclonal antibody (AAA213580 lot 1) used for IP at 20 ul/mg lysate. Cytokeratin-20 was also immunoprecipitated by rabbit anti-Cytokeratin-20 antibody BL1464B-2D4. For blotting immunoprecipitated Cytokeratin-20, AAA213580 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Cytokeratin-20 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HT-29 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Cytokeratin-20 recombinant monoclonal antibody (AAA213580 lot 1) used for IP at 20 ul/mg lysate. Cytokeratin-20 was also immunoprecipitated by rabbit anti-Cytokeratin-20 antibody BL1464B-2D4. For blotting immunoprecipitated Cytokeratin-20, AAA213580 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohiostchemistry)
(Detection of human Cytokeratin 20 in FFPE colon carcinoma by IHC. Antibody: Rabbit anti-Cytokeratin 20 recombinant monoclonal antibody (AAA213580 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human Cytokeratin 20 in FFPE colon carcinoma by IHC. Antibody: Rabbit anti-Cytokeratin 20 recombinant monoclonal antibody (AAA213580 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Cytokeratin 20 in FFPE HT-29 cells by ICC. Antibody: Rabbit anti-Cytokeratin 20 recombinant monoclonal antibody (AAA213580 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Cytokeratin 20 in FFPE HT-29 cells by ICC. Antibody: Rabbit anti-Cytokeratin 20 recombinant monoclonal antibody (AAA213580 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for KRT20 recombinant antibody
Cytokeratin 20 is a member of the keratin family of intermediate filament proteins responsible for the structural integrity of epithelial cells. Cytokeratin 20 is a type I cytokeratin, acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. [taken from NCBI Entrez Gene (Gene ID: 54474)].
Product Categories/Family for KRT20 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,487 Da
NCBI Official Full Name
keratin, type I cytoskeletal 20
NCBI Official Synonym Full Names
keratin 20
NCBI Official Symbol
KRT20
NCBI Official Synonym Symbols
K20; CD20; CK20; CK-20; KRT21
NCBI Protein Information
keratin, type I cytoskeletal 20; keratin-20; protein IT; cytokeratin 20; cytokeratin-20
UniProt Protein Name
Keratin, type I cytoskeletal 20
UniProt Gene Name
KRT20
UniProt Synonym Gene Names
CK-20; K20
UniProt Entry Name
K1C20_HUMAN
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Product Notes
The KRT20 krt20 (Catalog #AAA213580) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Cytokeratin 20 Recombinant Monoclonal Antibody [BLR105H] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 20 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the KRT20 krt20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 20, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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