ICC (Immunocytochemistry)
(Immunocytochemical labeling of delta1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal delta1-Catenin (a.a. 275-285), delta1-Catenin (Tyr-228), delta1-Catenin (Tyr-280), or delta1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.)
Mouse delta1-Catenin Monoclonal Antibody | anti-CTNND1 antibody
delta1-Catenin (Tyr-228), phospho-specific Antibody
Gene Names
CTNND1; CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
Reactivity
Human, Rat, Mouse
Applications
Immunocytochemistry, ELISA, Western Blot
Purity
Protein A chromatography
Synonyms
delta1-Catenin, Antibody; delta1-Catenin (Tyr-228), phospho-specific Antibody; delta1-Catenin (Tyr-228), phospho-specific; Catenin 4 228; anti-CTNND1 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M356
Specificity
The antibody detects a 110kDa* protein corresponding to the molecular mass of delta1-Catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells treated with pervanadate. This antibody does not detect this band after alkaline phosphatase treatment.
Purity/Purification
Protein A chromatography
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-CTNND1 antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Clone M356 was generated from a phospho-delta1-Catenin (Tyr-228) synthetic peptide containing amino acid residues around tyrosine 228 of human delta1-Catenin. This peptide sequence is highly conserved in rat and mouse delta1-Catenin.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of delta1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal delta1-Catenin (a.a. 275-285), delta1-Catenin (Tyr-228), delta1-Catenin (Tyr-280), or delta1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of delta1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal delta1-Catenin (a.a. 275-285), delta1-Catenin (Tyr-228), delta1-Catenin (Tyr-280), or delta1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.)
WB (Western Blot)
(Western blot analysis of delta1-Catenin phosphorylation in A431 cells stimulated with pervanadate (1mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-delta1-Catenin (a.a. 275-285) (lanes 1 & 2), anti-delta1-Catenin (Tyr-96) (lanes 3 & 4), anti-delta1-Catenin (Tyr-228) (lanes 5 & 6), anti-delta1-Catenin (Tyr-280) (lanes 7 & 8) or anti-delta1-Catenin (Tyr-302) (lanes 9 & 10).)
WB (Western Blot)
(Western blot analysis of delta1-Catenin phosphorylation in A431 cells stimulated with pervanadate (1mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-delta1-Catenin (a.a. 275-285) (lanes 1 & 2), anti-delta1-Catenin (Tyr-96) (lanes 3 & 4), anti-delta1-Catenin (Tyr-228) (lanes 5 & 6), anti-delta1-Catenin (Tyr-280) (lanes 7 & 8) or anti-delta1-Catenin (Tyr-302) (lanes 9 & 10).)
Related Product Information for anti-CTNND1 antibody
Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. delta1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. delta1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by delta1-Catenin, which can modulate RhoA, Rac and cdc42 activity. delta1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of delta1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in delta1-Catenin associated with the plasma membrane.
References
Fukumoto, Y. et al. (2008) Exp. Cell Res. 314:52.
Reynolds, A.B. & Roczniak-Ferguson, A. (2004) Oncogene 23:7947.
Mariner, D.J. et al. (2001) J. Biol. Chem. 276:28006.
Reynolds, A.B. & Roczniak-Ferguson, A. (2004) Oncogene 23:7947.
Mariner, D.J. et al. (2001) J. Biol. Chem. 276:28006.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
108,170 Da
NCBI Official Full Name
catenin delta-1 isoform 1ABC
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), delta 1
NCBI Official Symbol
CTNND1
NCBI Official Synonym Symbols
CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
NCBI Protein Information
catenin delta-1; p120 catenin; cadherin-associated Src substrate
UniProt Protein Name
Catenin delta-1
UniProt Gene Name
CTNND1
UniProt Synonym Gene Names
KIAA0384; CAS; p120(ctn)
UniProt Entry Name
CTND1_HUMAN
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Product Notes
The CTNND1 ctnnd1 (Catalog #AAA71595) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The delta1-Catenin (Tyr-228), phospho-specific Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's delta1-Catenin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CTNND1 ctnnd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "delta1-Catenin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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