IF (Immunofluorescence)
(Immunofluorescence of E2F1(green) in Hela cells using E2F1 Rabbit mAb at dilution 1:50, and DAPI(blue))
Rabbit E2F1 Monoclonal Antibody | anti-E2F1 antibody
Recombinant E2F1 Monoclonal Antibody
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
E2F1, Antibody; Recombinant E2F1 Monoclonal Antibody; E2F-1; RBAP1; RBBP3; RBP3; E2F1; anti-E2F1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
R03-5G3
Purity/Purification
Affinity Purified
Form/Format
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Concentration
300ug/mL (varies by lot)
Applicable Applications for anti-E2F1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide of human E2F1
Research Area
Cancer, Epigenetics and Nuclear Signaling
Preparation and Storage
Storage: -20 degree C/One year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence of E2F1(green) in Hela cells using E2F1 Rabbit mAb at dilution 1:50, and DAPI(blue))
IF (Immunofluorescence)
(Immunofluorescence of E2F1(green) in Hela cells using E2F1 Rabbit mAb at dilution 1:50, and DAPI(blue))
IHC (Immunohiostchemistry)
(Immunohistochemistry of E2F1 in paraffin-embedded Human tonsil using E2F1 Rabbit mAb at dilution 1:20)
IHC (Immunohiostchemistry)
(Immunohistochemistry of E2F1 in paraffin-embedded Human tonsil using E2F1 Rabbit mAb at dilution 1:20)
WB (Western Blot)
(Western blot detection of E2F1 in C6,3T3 cell lysates using E2F1 Rabbit mAb(1:1000 diluted).Predicted band size:47kDa.Observed band size:70kDa.)
WB (Western Blot)
(Western blot detection of E2F1 in C6,3T3 cell lysates using E2F1 Rabbit mAb(1:1000 diluted).Predicted band size:47kDa.Observed band size:70kDa.)
Related Product Information for anti-E2F1 antibody
The protein encoded by this gene is member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain.
Product Categories/Family for anti-E2F1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,920 Da
NCBI Official Full Name
transcription factor E2F1
NCBI Official Synonym Full Names
E2F transcription factor 1
NCBI Official Symbol
E2F1
NCBI Official Synonym Symbols
RBP3; E2F-1; RBAP1; RBBP3
NCBI Protein Information
transcription factor E2F1; PBR3; RBAP-1; RBBP-3; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1
UniProt Protein Name
Transcription factor E2F1
UniProt Gene Name
E2F1
UniProt Synonym Gene Names
RBBP3; E2F-1; RBAP-1; RBBP-3
UniProt Entry Name
E2F1_HUMAN
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Product Notes
The E2F1 e2f1 (Catalog #AAA178845) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant E2F1 Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's E2F1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the E2F1 e2f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E2F1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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