IP (Immunoprecipitation)
(EBAG9 was immunoprecipitated using:Lane A:0.5 mg THP-1 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate2 uL anti-EBAG9 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EBAG9 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size: 37 kDa)
Rabbit anti-Human EBAG9 Monoclonal Antibody | anti-EBAG9 antibody
Recombinant Anti-EBAG9 Antibody, Rabbit Monoclonal
Gene Names
EBAG9; EB9; PDAF
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A
Synonyms
EBAG9, Antibody; Recombinant Anti-EBAG9 Antibody, Rabbit Monoclonal; RCAS1/EBAG9 Antibody, Rabbit MAb; estrogen receptor binding site associated, antigen, 9; Anti-EB9 Antibody; Anti-PDAF Antibody; anti-EBAG9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
019
Specificity
Human RCAS1/EBAG9
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-EBAG9 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human RCAS1/EBAG9 Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human RCAS1/EBAG9 (rh RCAS1/EBAG9).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(EBAG9 was immunoprecipitated using:Lane A:0.5 mg THP-1 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate2 uL anti-EBAG9 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EBAG9 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size: 37 kDa)
IP (Immunoprecipitation)
(EBAG9 was immunoprecipitated using:Lane A:0.5 mg THP-1 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate2 uL anti-EBAG9 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EBAG9 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size: 37 kDa)
WB (Western Blot)
(Anti-EBAG9 rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:37 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-EBAG9 rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:37 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-EBAG9 antibody
RCAS1, also known as EBAG9, is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. RCAS1 gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of RCAS1 gene. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for RCAS1 gene. EBAG9 may participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
References
Ohshima K. et al., 2001, Clin Exp Immunol. 123 (3): 481-6. Ikeda K. et al., 2000, Biochem Biophys Res Commun. 273 (2): 654-60. Nakashima M. et al., 1999, Nat Med. 5 (8): 938-42.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
213
NCBI Official Full Name
Receptor-binding cancer antigen expressed on SiSo cells
NCBI Official Synonym Full Names
estrogen receptor binding site associated, antigen, 9
NCBI Official Symbol
EBAG9
NCBI Official Synonym Symbols
EB9; PDAF
NCBI Protein Information
receptor-binding cancer antigen expressed on SiSo cells; cancer-associated surface antigen RCAS1; estrogen receptor-binding fragment-associated gene 9 protein
UniProt Protein Name
Receptor-binding cancer antigen expressed on SiSo cells
UniProt Gene Name
EBAG9
UniProt Synonym Gene Names
RCAS1
UniProt Entry Name
RCAS1_HUMAN
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Product Notes
The EBAG9 ebag9 (Catalog #AAA255715) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-EBAG9 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EBAG9 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the EBAG9 ebag9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EBAG9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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