WB (Western Blot)
(Western blot analysis of non-reduced A431 cell lysate probed with anti-EGF Receptor (1ug/ml))
Mouse anti-Human EGFR Monoclonal Antibody | anti-EGFR antibody
EGFR antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
Purity
EGFR antibody was purified by Protein G chromatography.
Synonyms
EGFR, Antibody; EGFR antibody; Monoclonal EGFR; Anti-EGFR; Epidermal Growth FactorEgf receptor; panitumumab; epidermal growth factor receptor; egfr; v-erb-b homolog; egf-r; erbb1; anti-EGFR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LA1
Specificity
Human
Purity/Purification
EGFR antibody was purified by Protein G chromatography.
Form/Format
Lyophilized from 250ul 0.1M tris-glycine, pH 7.4, 0.15M NaCl.
Sequence Length
1167
Applicable Applications for anti-EGFR antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Biological Significance
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGF alpha). EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity.
Immunogen
EGFR antibody was raised in mouse using human A431 membrane protein as the immunogen.
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of non-reduced A431 cell lysate probed with anti-EGF Receptor (1ug/ml))
WB (Western Blot)
(Western blot analysis of non-reduced A431 cell lysate probed with anti-EGF Receptor (1ug/ml))
ICC (Immunocytochemistry)
(A431 cells were stained with 2ug/ml of anti-EGF Receptor (neutralizing), clone LA1, Alexa Fluor 488 conjugate (Panel A) or Alexa Fluor 488-conjugated Normal Mouse IgG (Panel B) (green) and DAPI (blue).)
ICC (Immunocytochemistry)
(A431 cells were stained with 2ug/ml of anti-EGF Receptor (neutralizing), clone LA1, Alexa Fluor 488 conjugate (Panel A) or Alexa Fluor 488-conjugated Normal Mouse IgG (Panel B) (green) and DAPI (blue).)
Related Product Information for anti-EGFR antibody
Mouse monoclonal EGFR antibody
Product Categories/Family for anti-EGFR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
180 kDa (predicted detection band MW)
NCBI Official Full Name
epidermal growth factor receptor
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
NCBI Protein Information
epidermal growth factor receptor
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN
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Product Notes
The EGFR egfr (Catalog #AAA74528) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EGFR antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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