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WB (Western Blot)
(Detection of mouse EGFR by western blot. Samples: Whole cell lysate (10 ug) from AML12, NIH 3T3, and mIMCD-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (AAA213665 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
EGFR recombinant antibody
Rabbit anti-EGFR Recombinant Monoclonal Antibody [BLR252L]
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
EGFR, Recombinant Antibody; Rabbit anti-EGFR Recombinant Monoclonal Antibody [BLR252L]; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; PIG61; NISBD2; receptor tyrosine-protein kinase erbB-1; HER1; ERBB; epidermal growth factor receptor tyrosine kinase domain; epidermal growth factor receptor; cell proliferation-inducing protein 61; cell growth inhibiting protein 40; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; mENA; ERBB1; Epidermal growth factor receptor; EGFR recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR252L
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for EGFR recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1160 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse EGFR by western blot. Samples: Whole cell lysate (10 ug) from AML12, NIH 3T3, and mIMCD-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (AAA213665 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse EGFR by western blot. Samples: Whole cell lysate (10 ug) from AML12, NIH 3T3, and mIMCD-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (AAA213665 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human EGFR by western blot. Samples: Whole cell lysate (10 ug) from GaMG, HeLa, OVCAR-8, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (AAA213665 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human EGFR by western blot. Samples: Whole cell lysate (10 ug) from GaMG, HeLa, OVCAR-8, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (AAA213665 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
ICC (Immunocytochemistry)
(Detection of human EGFR by immunocytochemistry. Sample: FFPE section of A-431 cells. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (AAA213665 lot 1) used for IP at 6 ul per reaction. EGFR was also immunoprecipitated by a second antibody against a different epitope of EGFR (BL-2664A-1G11). For blotting immunoprecipitated EGFR, AAA213665 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
ICC (Immunocytochemistry)
(Detection of human EGFR by immunocytochemistry. Sample: FFPE section of A-431 cells. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (AAA213665 lot 1) used for IP at 6 ul per reaction. EGFR was also immunoprecipitated by a second antibody against a different epitope of EGFR (BL-2664A-1G11). For blotting immunoprecipitated EGFR, AAA213665 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
FCM/FACS (Flow Cytometry)
(Detection of mouse EGFR (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (AAA213665) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of mouse EGFR (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (AAA213665) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human EGFR (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (AAA213665) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human EGFR (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (AAA213665) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for EGFR recombinant antibody
The epidermal growth factor receptor (EGFR) is a membrane spanning tyrosine kinase receptor for epidermal growth factor (EGF) and other ligands of the EGF family such as TGF-alpha, amphiregulin, betacellulin, epiregulin and heparin-binding EGF-like growth factor. Upon ligand binding, the EGFR homodimerizes or heterodimerizes with other ErbB receptor family members and tranduces a downstream signal via the ras-raf-mitogen activated protein kinase pathway or the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. Signals from the EGFR function to influence cell proliferation, differentiation, and development.
Product Categories/Family for EGFR recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
134,277 Da
NCBI Official Full Name
epidermal growth factor receptor isoform a
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61
NCBI Protein Information
epidermal growth factor receptor; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN
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Product Notes
The EGFR egfr (Catalog #AAA213665) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-EGFR Recombinant Monoclonal Antibody [BLR252L] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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