Rabbit anti-Human eIF4EBP1-T70 Monoclonal Antibody | anti-EIF4EBP1 antibody

Phospho-eIF4EBP1-T70 Rabbit mAb

Cat. Num. AAA283141

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity purification
• Synonyms: eIF4EBP1-T70, Antibody; Phospho-eIF4EBP1-T70 Rabbit mAb; BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-T70; anti-EIF4EBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
• Sequence: TKTPPR

• Applicable Applications for anti-EIF4EBP1 antibody: ELISA, WB (Western Blot)
• Cross Reactivity: Human, Mouse
• Immunogen: A synthetic phosphorylated peptide around T70 of human eIF4EBP1 (NP_004086.1).
• Modification: Phospho T70
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

WB (Western Blot)

(Western blot analysis of lysates from NIH/3T3 cells, using Phospho-eIF4EBP1-T70 Rabbit mAb (AAA283141) at1:2000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37? for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

WB (Western Blot)

(Western blot analysis of various lysates using Phospho-eIF4EBP1-T70 Rabbit mAb (AAA283141) at 1:2000 dilution. 293T cells were treated by 10% FBS at 37? for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37? for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

Related Product Information for anti-EIF4EBP1 antibody

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Product Categories/Family for anti-EIF4EBP1 antibody

Epigenetics Nuclear Signaling; Translation Control; Regulation of eIF4 and p70 S6 Kinase; Protein phosphorylation; Signal Transduction; PI3K-Akt Signaling Pathway; mTOR Signaling Pathway; Endocrine Metabolism; AMPK Signaling Pathway; Insulin Receptor Sign

NCBI and Uniprot Product Information

• NCBI GI #: 4758258
• NCBI GeneID: 1978
• NCBI Accession #: NP_004086.1
• NCBI GenBank Nucleotide #: NM_004095.3
• UniProt Accession #: Q13541; Q6IBN3; B2R502; D3DSW8
• Molecular Weight: Calculated MW: 13kDa; Observed MW: 15-20kDa
• UniProt Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
• UniProt Gene Name: EIF4EBP1
• UniProt Synonym Gene Names: 4E-BP1; eIF4E-binding protein 1; PHAS-I
• UniProt Entry Name: 4EBP1_HUMAN