DB (Dot Blot)
(Dot blot analysis of anti-EIF4EBP1-pS111 Phospho-specific Pab (RB13351) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit Phospho-EIF4EBP1 (S111) Polyclonal Antibody | anti-EIF4EBP1 antibody
Phospho-EIF4EBP1 (S111) Antibody
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
ELISA, Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-EIF4EBP1 (S111), Antibody; Phospho-EIF4EBP1 (S111) Antibody; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I; EIF4EBP1; anti-EIF4EBP1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EIF4EBP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S111 of human EIF4EBP1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.29 (varies by lot)
Sequence Length
118
Applicable Applications for anti-EIF4EBP1 antibody
ELISA, DB (Dot Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex:
hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of anti-EIF4EBP1-pS111 Phospho-specific Pab (RB13351) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
DB (Dot Blot)
(Dot blot analysis of anti-EIF4EBP1-pS111 Phospho-specific Pab (RB13351) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Related Product Information for anti-EIF4EBP1 antibody
EIF4EBP1 is a member of a family of translation repressor proteins. This protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of EIF4EBP1 with eIF4E inhibits complex assembly and represses translation. EIF4EBP1 is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Product Categories/Family for anti-EIF4EBP1 antibody
References
Fonseca,B.D., J. Biol. Chem. 282 (34), 24514-24524 (2007)
Armengol,G., Cancer Res. 67 (16), 7551-7555 (2007)
Armengol,G., Cancer Res. 67 (16), 7551-7555 (2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12580
NCBI Official Full Name
eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
EIF4EBP1
NCBI Official Synonym Symbols
BP-1; 4EBP1; 4E-BP1; PHAS-I
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Gene Name
EIF4EBP1
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I
UniProt Entry Name
4EBP1_HUMAN
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Product Notes
The EIF4EBP1 eif4ebp1 (Catalog #AAA288214) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-EIF4EBP1 (S111) Antibody reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-EIF4EBP1 (S111) can be used in a range of immunoassay formats including, but not limited to, ELISA, DB (Dot Blot). Researchers should empirically determine the suitability of the EIF4EBP1 eif4ebp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-EIF4EBP1 (S111), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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