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Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Mouse Emerin Monoclonal Antibody (EMD/2168) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse Emerin Monoclonal Antibody | anti-EMD antibody
Emerin (Papillary Thyroid Carcinoma and EDMD Marker)
Gene Names
EMD; STA; EDMD; LEMD5
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Synonyms
Emerin, Antibody; Emerin (Papillary Thyroid Carcinoma and EDMD Marker); EMD; Emerin; Emery Dreifuss muscular dystrophy (EDMD); STA; anti-EMD antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
EMD/2168
Specificity
Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in papillary thyroid carcinoma.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
254
Applicable Applications for anti-EMD antibody
Immunohistochemistry (IHC) Formalin
Application Notes
IHC-F: 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Immunogen
Recombinant human Emerin protein fragment (around aa 56-167)
Cellular Localization
Nuclear Membrane
Hu-chromosome Location
Xq28
Positive Control
K-562, HeLa or Jurkat cells. Kidney or Skin
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Mouse Emerin Monoclonal Antibody (EMD/2168) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Mouse Emerin Monoclonal Antibody (EMD/2168) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis Purified Emerin Mouse Monoclonal Antibody (EMD/2168).Confirmation of Integrity and Purity of Antibody)
SDS-PAGE
(SDS-PAGE Analysis Purified Emerin Mouse Monoclonal Antibody (EMD/2168).Confirmation of Integrity and Purity of Antibody)
WB (Western Blot)
(Western Blot Analysis of human HeLa Cell lysate using Emerin Mouse Monoclonal Antibody (EMD/2168).)
WB (Western Blot)
(Western Blot Analysis of human HeLa Cell lysate using Emerin Mouse Monoclonal Antibody (EMD/2168).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2168).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2168).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2168).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2168).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2168).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2168).)
References
Bione, S., Maestrini, E., Rivella, S., Mancini, M., Regis, S., Romeo, G. and Toniolo, D. 1994. Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy. Nat. Genet. 8: 323-327.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37kDa
NCBI Official Full Name
emerin
NCBI Official Synonym Full Names
emerin
NCBI Official Symbol
EMD
NCBI Official Synonym Symbols
STA; EDMD; LEMD5
NCBI Protein Information
emerin
UniProt Protein Name
Emerin
UniProt Gene Name
EMD
UniProt Synonym Gene Names
EDMD; STA
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Product Notes
The EMD emd (Catalog #AAA23899) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Emerin (Papillary Thyroid Carcinoma and EDMD Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's Emerin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin. IHC-F: 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the EMD emd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Emerin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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