IHC (Immunohistochemistry)
(Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Mouse Endostatin Monoclonal Antibody | anti-COL18A1 antibody
Mouse Monoclonal (IgG) to Mouse Endostatin
Gene Names
COL18A1; KS; KNO; KNO1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Thiophilic adsorption
Synonyms
Endostatin, Antibody; Mouse Monoclonal (IgG) to Mouse Endostatin; Anti-Endostatin Antibody IHC-plus; COL18A1; Collagen; type XVIII; alpha 1; Collagen alpha-1(XVIII) chain; Endostatin; KNO; KNO1; KS; Multi-functional protein MFP; Antiangiogenic agent; Collagen alpha 1(xviii); Knobloch syndrome; type 1; Mouse Endostatin; anti-COL18A1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Specificity
Recognizes mouse endostatin, Mr 22kD, an unidentified protein is also weakly detected, Mr 54kD. Detects human protein in IHC-FFPE. It is predicted that the antibody will also detect the rat protein. Cross-reactivity is predicted based on sequence homology. The antibody has not been specifically tested with rat samples at this time.
Purity/Purification
Thiophilic adsorption
Form/Format
50 mM sodium phosphate, pH 8.0, 0.05% sodium azide, before the addition of glycerol to 30%. Sourced in Ascites.
Sequence Length
1516
Applicable Applications for anti-COL18A1 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Mouse
Taget
Endostatin
Immunogen Description
Full-length recombinant murine endostatin.
Immunogen
Endostatin antibody was raised against full-length recombinant murine endostatin.
Usage Summary
Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245224 was determined to be 1:100. Western Blot: 1:500-1:2000. Dectects endostatin in mouse liver cell lysates to which 50ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS245224. Proteins were visualized using an IgG (HRP) goat anti-mouse and chemiluminescence detection system.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
IHC (Immunohistochemistry)
(Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
IHC (Immunohistochemistry)
(Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Related Product Information for anti-COL18A1 antibody
Endostatin, which corresponds to the C-terminal fragment of collagen XVIII, is a potent inhibitor of endothelial cell proliferation, migration and angiogenesis. Recent study shows that endostatin induces tyrosine kinase activity and enhanced apoptosis in FGF-treated endothelial cells. Endostatin is currently being used in Phase I trails as an antitumor agent.
Endostatin is the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants.
Endostatin is the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-COL18A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
collagen alpha-1(XVIII) chain isoform 1 preproprotein
NCBI Official Synonym Full Names
collagen, type XVIII, alpha 1
NCBI Official Symbol
COL18A1
NCBI Official Synonym Symbols
KS; KNO; KNO1
NCBI Protein Information
collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; endostatin; antiangiogenic agent; multi-functional protein MFP
UniProt Protein Name
Collagen alpha-1(XVIII) chain
UniProt Gene Name
COL18A1
UniProt Entry Name
COIA1_HUMAN
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Product Notes
The COL18A1 col18a1 (Catalog #AAA51802) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal (IgG) to Mouse Endostatin reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Endostatin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the COL18A1 col18a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Endostatin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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