Mouse anti-Human EPHA2 Monoclonal Antibody | anti-EPHA2 antibody

anti-human EphA2 receptor tyrosine kinase (EphA2) monoclonal antibody

Cat. Num. AAA71474

• Gene Names: EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Flow Cytometry, Functional Assay
• Purity: Protein G
• Synonyms: EPHA2, Antibody; anti-human EphA2 receptor tyrosine kinase (EphA2) monoclonal antibody; Eph receptor tyrosine kinase A2; anti-EPHA2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: K?-5H5
• Specificity: anti-human EphA2
• Purity/Purification: Protein G
• Form/Format: Purified
• Sequence Length: 976

• Applicable Applications for anti-EPHA2 antibody: IF (Immunofluorescence), ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
• Buffer: phosphate buffered saline, pH 7.2
• Immunogen: genetic immunisation with cDNA encoding human EphA2
• Selection: based on recognition of the complete native protein expressed on transfected mammalian cells
• Immunogen: cDNA
• Preparation and Storage: Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)

Application Data

(Fig.3: SDS-PAGE analysis of purified K?-5H5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified K?-5H5 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)

Application Data

(Fig.2: Spectral Confocal Microscopy of CHO cells using K?-5H5 Cat.# GM-0901. CHO cells were transiently transfected with an expression vector encoding EphA2. Binding of K?-5H5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).)

Application Data

(Fig.1: FACS analysis of BOSC23 cells using K?-5H5 Cat.# GM-0901. BOSC23 cells were transiently transfected with an expression vector encoding either EphA2 (red curve) or an irrelevant protein (control transfectant). Binding of K?-5H5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with EphA2 transfected cells.)

Related Product Information for anti-EPHA2 antibody

Background: EphA2 (Eph receptor tyrosine kinase A2) belongs to the Eph tyrosine receptor familiy, the largest receptor tyrosine kinase family of transmembrane proteins. It encodes a 130 kDa transmembrane protein which is primarily found in adult human epithelial cells (1). Eph receptors and their ephrin ligands are important mediators of cell-cell communication and play roles in embryonic patterning, neuronal targeting, and vascular development during normal embryogenesis (2,3). The Eph family of receptor tyrosine kinases is frequently overexpressed in a wide variety of cancers and tumor cell lines. In particular, EphA2 is overexpressed in prostate, lung and colon cancers and 40% of breast cancers and it represent an attractive potential target for drug design (3,4).

References

Lindberg RA and Hunter T (1990). cDNA cloning and characterization of eck, an epithelial cell receptor protein- tyrosine kinase in the Eph/elk family of protein kinases. Mol Cell Biol 10:6316–24.
Brantley-Sieders d, Schmidt s, Parker M and Chen J (2004). Eph receptor tyrosine kinases in tumor and tumor microenvironment. Curr Pharm Des 10(27):3431-42
Nakamoto M and Bergmann AD (2002). Diverse roles for the Eph family of receptor tyrosine kinases in carcinogenesis. Microsc Res Tech 1; 59(1):58-67
Ireton RC and Chen J (2005). EphA2 receptor tyrosine kinase as a promising target for cancer therapeutics. Curr Canc Drug Targets 5(3): 149-57

NCBI and Uniprot Product Information

• NCBI GI #: 32967311
• NCBI GeneID: 1969
• NCBI Accession #: NP_004422.2
• NCBI GenBank Nucleotide #: NM_004431.3
• UniProt Accession #: P29317; Q8N3Z2
• Molecular Weight: 108,266 Da
• NCBI Official Full Name: ephrin type-A receptor 2
• NCBI Official Synonym Full Names: EPH receptor A2
• NCBI Official Symbol: EPHA2
• NCBI Official Synonym Symbols: ECK; CTPA; ARCC2; CTPP1; CTRCT6
• NCBI Protein Information: ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase
• UniProt Gene Name: EPHA2
• UniProt Synonym Gene Names: ECK
• UniProt Entry Name: EPHA2_HUMAN