ICC (Immunocytochemistry)
(Immunocytochemical labeling of EphA2 in aldehyde fixed human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-EphA2 (EM0881). The antibody was detected using goat anti-mouse DyLight 594.)
Mouse anti-Human EphA2 Monoclonal Antibody | anti-EphA2 antibody
EphA2 (Extracellular region) M088 Antibody
Gene Names
EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
Reactivity
Human
Applications
Immunocytochemistry, Immunoprecipitation, ELISA
Purity
Protein G chromatography
Synonyms
EphA2, Antibody; EphA2 (Extracellular region) M088 Antibody; EphA2 M088; anti-EphA2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M088
Specificity
Clone M088 mouse monoclonal antibody detects native human EphA2, as well as aldehyde-fixed EphA2. The antibody works for staining in immunocytochemistry, capture and detection in ELISA, and native immunoprecipitation assays.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-EphA2 antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), ELISA
Immunogen
Clone (M088) was generated from a recombinant protein that included the extracellular region of human EphA2 protein.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of EphA2 in aldehyde fixed human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-EphA2 (EM0881). The antibody was detected using goat anti-mouse DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of EphA2 in aldehyde fixed human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-EphA2 (EM0881). The antibody was detected using goat anti-mouse DyLight 594.)
Standard Curve (Sample)
(Representative Standard Curve using mouse monoclonal anti-EphA2 (EM0881) for ELISA capture of human recombinant EphA2 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.)
Standard Curve (Sample)
(Representative Standard Curve using mouse monoclonal anti-EphA2 (EM0881) for ELISA capture of human recombinant EphA2 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.)
Related Product Information for anti-EphA2 antibody
The EpHfamily of receptor tyrosine kinases and their Ephrin ligands are important for cell positioning and morphogenesis during development. EpHreceptors are classified into 10 EphA and 6 EphB receptors, which preferentially bind to the type A and type B ephrins, respectively. Ephrin type-A receptor 2 (EphA2), also known as epithelial cell kinase (Eck), binds the ephrin A1 (EFNA1) ligand, and has roles in neuronal development and repair, as well as carcinogenesis. EphA2 receptor has an N-terminal ligand-binding domain followed by a cysteine-rich domain with an epidermal growth factor-like motif and two fibronectin type-III repeats in the extracellular region, and a sterile alpha motif (SAM), and a PDZ domain-binding motif in the intracellular region. EphA2 is expressed in many types of cancers, including breast, colon, bladder, gastric, and glioblastoma. In bladder cancers, EphA2 may be activated by progranulin leading to phosphorylation at Ser-897 and bladder tumorigenesis. EphA2 may be an important therapeutic target and biomarker for several types of cancer.
References
Neill, T. et al. (2016) J Cell Biol. 215(5):687.
Zhou, Y. & Sakurai, H. (2017) Biol Pharm Bull. 40(10):1616.
Zhou, Y. & Sakurai, H. (2017) Biol Pharm Bull. 40(10):1616.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
108,266 Da
NCBI Official Full Name
ephrin type-A receptor 2
NCBI Official Synonym Full Names
EPH receptor A2
NCBI Official Symbol
EPHA2
NCBI Official Synonym Symbols
ECK; CTPA; ARCC2; CTPP1; CTRCT6
NCBI Protein Information
ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase
UniProt Protein Name
Ephrin type-A receptor 2
UniProt Gene Name
EPHA2
UniProt Synonym Gene Names
ECK
UniProt Entry Name
EPHA2_HUMAN
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Product Notes
The EphA2 epha2 (Catalog #AAA71623) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EphA2 (Extracellular region) M088 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EphA2 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), ELISA. Researchers should empirically determine the suitability of the EphA2 epha2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EphA2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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