WB (Western Blot)
(ERCC1 Antibody (C-term) western blot analysis in Hela,A549 cell line lysates (35ug/lane).This demonstrates the ERCC1 antibody detected the ERCC1 protein (arrow).)
Mouse anti-Human ERCC1 Monoclonal Antibody | anti-ERCC1 antibody
ERCC1 Antibody (C-term)
Gene Names
ERCC1; UV20; COFS4; RAD10
Reactivity
Human
Applications
ELISA, Western Blot
Synonyms
ERCC1, Antibody; ERCC1 Antibody (C-term); DNA excision repair protein ERCC-1; ERCC1; anti-ERCC1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgM,k
Clone Number
752CT13.2.5
Specificity
This ERCC1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 268-297 amino acids from the C-terminal region of human ERCC1.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 1.12 (varies by lot)
Sequence Positions
268-297
Sequence Length
273
Applicable Applications for anti-ERCC1 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(ERCC1 Antibody (C-term) western blot analysis in Hela,A549 cell line lysates (35ug/lane).This demonstrates the ERCC1 antibody detected the ERCC1 protein (arrow).)
WB (Western Blot)
(ERCC1 Antibody (C-term) western blot analysis in Hela,A549 cell line lysates (35ug/lane).This demonstrates the ERCC1 antibody detected the ERCC1 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from A549, T47D cell line (from left to right), using ERCC1 Antibody (C-term). AAA283586 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A549, T47D cell line (from left to right), using ERCC1 Antibody (C-term). AAA283586 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(All lanes : Anti-ERCC1 Antibody (C-term) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: T47D whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 33 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-ERCC1 Antibody (C-term) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: T47D whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 33 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-ERCC1 antibody
Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
Product Categories/Family for anti-ERCC1 antibody
References
van Duin M., et al. Cell 44:913-923(1986).
Hoeijmakers J.H.J., et al. Cold Spring Harb. Symp. Quant. Biol. 51:91-101(1986).
Yu J.J., et al. Mutat. Res. 382:13-20(1997).
Hisatomi H., et al. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Hoeijmakers J.H.J., et al. Cold Spring Harb. Symp. Quant. Biol. 51:91-101(1986).
Yu J.J., et al. Mutat. Res. 382:13-20(1997).
Hisatomi H., et al. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32562
NCBI Official Full Name
DNA excision repair protein ERCC-1 isoform 3
NCBI Official Synonym Full Names
excision repair cross-complementation group 1
NCBI Official Symbol
ERCC1
NCBI Official Synonym Symbols
UV20; COFS4; RAD10
NCBI Protein Information
DNA excision repair protein ERCC-1
UniProt Protein Name
DNA excision repair protein ERCC-1
UniProt Gene Name
ERCC1
UniProt Entry Name
ERCC1_HUMAN
Similar Products
Product Notes
The ERCC1 ercc1 (Catalog #AAA283586) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 268-297. The ERCC1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERCC1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ERCC1 ercc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERCC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.