SDS-PAGE
(SDS-PAGE Analysis of Purified Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3373) (unconjugated). Confirmation of Integrity and Purity of Antibody)
Mouse anti-Human Estrogen Receptor, alpha Monoclonal Antibody | anti-ESR1 antibody
Estrogen Receptor, alpha (Marker of Estrogen Dependence)
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified Ab conjugated to PE
Synonyms
Estrogen Receptor, alpha, Antibody; Estrogen Receptor, alpha (Marker of Estrogen Dependence); Estrogen Receptor alpha delta 4*5, 6, 7*/654 isoform; Estrogen Receptor alpha delta 4 +49 isoform; Nuclear receptor subfamily 3 group A member 1; anti-ESR1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
ER506
Specificity
This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
Purity/Purification
Purified Ab conjugated to PE
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
Sequence Length
595
Applicable Applications for anti-ESR1 antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Immunogen
Recombinant fragment (around aa 2-185) of human Estrogen Receptor alpha protein (exact sequence is proprietary)
Cellular Localization
Cell membrane, Cytoplasm, Golgi apparatus, Nucleus
Positive Control
T47D, MCF-7 cells. Breast or Endometrial Carcinoma.
Research Areas
Breast Cancer, Cancer, Cardiovascular, Infectious Disease, Ovarian Cancer, Signal Transduction, Transcription Factors
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE Analysis of Purified Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3373) (unconjugated). Confirmation of Integrity and Purity of Antibody)
SDS-PAGE
(SDS-PAGE Analysis of Purified Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3373) (unconjugated). Confirmation of Integrity and Purity of Antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometry for human ER-alpha on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-alpha Monoclonal Antibody (ER506).)
FCM/FACS (Flow Cytometry)
(Flow Cytometry for human ER-alpha on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-alpha Monoclonal Antibody (ER506).)
References
Zafrani B, et. al. Histopathology 2000; 37(6), 536545. Harvey JM, et. al. Journal of Clinical Oncology 1999; 17(5), 14741481.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~67kDa
NCBI Official Full Name
estrogen receptor isoform 1
NCBI Official Synonym Full Names
estrogen receptor 1
NCBI Official Symbol
ESR1
NCBI Official Synonym Symbols
ER; ESR; Era; ESRA; ESTRR; NR3A1
NCBI Protein Information
estrogen receptor
UniProt Protein Name
Estrogen receptor
UniProt Gene Name
ESR1
UniProt Synonym Gene Names
ESR; NR3A1; ER
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Product Notes
The ESR1 esr1 (Catalog #AAA62969) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Estrogen Receptor, alpha (Marker of Estrogen Dependence) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Estrogen Receptor, alpha can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ESR1 esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Estrogen Receptor, alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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