Mouse anti-Human Fascin-1 Monoclonal Antibody | anti-FSCN1 antibody

Fascin-1 (Reed-Sternberg Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62794

• Gene Names: FSCN1; HSN; SNL; p55; FAN1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: Fascin-1, Antibody; Fascin-1 (Reed-Sternberg Cell Marker) Mouse Monoclonal Antibody; 55kDa actin-bundling protein; FAN1; Fascin homolog 1; Fascin1; FSCN1; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); SNL; anti-FSCN1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: FSCN1/417
• Specificity: Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 493

• Applicable Applications for anti-FSCN1 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cytoplasmic
• Immunogen: Full length recombinant human FSCN1 protein
• Hu-Chromosome Location: 7p22.1
• Positive Control: HeLa, MCF-7, PC-3 or BEWO cells. Hodgkin's lymphoma, Ovarian or Testicular Carcinoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

WB (Western Blot)

(Western Blot of HeLa Cell Lysate using Fascin-1 Monoclonal Antibody (FSN1/417))

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Fascin-1 Monoclonal Antibody (FSCN1/417))

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Fascin-1 Monoclonal Antibody (FSCN1/417))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Fascin-1 Monoclonal Antibody (FSCN1/417))

References

Yamashiro-Matsumura S and Matsumura F. J Biol Chem 1985; 260(8): 5087. Yamashro-Matsumura S and Matsumura F. J Cell Biol 1986; 103:631. Duh F-M, et al. DNA Cell Biol 1994; 13(8):821

NCBI and Uniprot Product Information

• NCBI GI #: 4507115
• NCBI GeneID: 6624
• NCBI Accession #: NP_003079.1
• NCBI GenBank Nucleotide #: NM_003088.3
• UniProt Accession #: Q16658; Q96IC5; Q96IH1; Q9BRF1; A6NI89; B2RE97
• Molecular Weight: 55kDa
• NCBI Official Full Name: fascin
• NCBI Official Synonym Full Names: fascin actin-bundling protein 1
• NCBI Official Symbol: FSCN1
• NCBI Official Synonym Symbols: HSN; SNL; p55; FAN1
• NCBI Protein Information: fascin
• UniProt Protein Name: Fascin
• UniProt Gene Name: FSCN1
• UniProt Synonym Gene Names: FAN1; HSN; SNL
• UniProt Entry Name: FSCN1_HUMAN