IF (Immunofluorescence)
(ICC/IF analysis of FSCN1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FSCN1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human FSCN1 Monoclonal Antibody | anti-FSCN1 antibody
FSCN1 antibody
Gene Names
FSCN1; HSN; SNL; p55; FAN1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
FSCN1, Antibody; FSCN1 antibody; Fascin; FAN1; HSN; SNL; anti-FSCN1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT13D2
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
493
Applicable Applications for anti-FSCN1 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human FSCN1 (1-493aa) purified from E. coli
Clone Note
Anti-human FSCN1 mAb, clone AT13D2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FSCN1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of FSCN1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FSCN1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of FSCN1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FSCN1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FSCN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : THP-1 cell lysate)
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FSCN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : THP-1 cell lysate)
Related Product Information for anti-FSCN1 antibody
FSCN1 is an actin-bundling protein that provides rigidity to filopodial bundles to efficiently push the membrane forward during cytoskeleton remodeling and cell migration. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. And this protein is absent from most normal epithelia yet is upregulated in multiple forms of human carcinoma, where its expression correlates clinically with a poor prognosis. FSCN1 expression has been localized to neurons, glial cells, and endothelial cells.
Product Categories/Family for anti-FSCN1 antibody
References
Wu D., et al. (2010) Ann Oncol. 21 (3): 589-596. ; Shin H., et al. (2009) Phys Rev Left. 103 (23): 238102.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,530 Da
NCBI Official Full Name
fascin
NCBI Official Synonym Full Names
fascin actin-bundling protein 1
NCBI Official Symbol
FSCN1
NCBI Official Synonym Symbols
HSN; SNL; p55; FAN1
NCBI Protein Information
fascin
UniProt Protein Name
Fascin
UniProt Gene Name
FSCN1
UniProt Synonym Gene Names
FAN1; HSN; SNL
Similar Products
Product Notes
The FSCN1 fscn1 (Catalog #AAA48188) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FSCN1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FSCN1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the FSCN1 fscn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FSCN1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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