Mouse anti-Human FOXA1/HNF3A Monoclonal Antibody | anti-FOXA1 antibody

FOXA1/HNF3A

Cat. Num. AAA215015

• Gene Names: FOXA1; HNF3A; TCF3A
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: FOXA1/HNF3A, Antibody; FOXA1/HNF3A; Forkhead box protein A1 (FOXA1); Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A); Transcription factor 3A (TCF-3A or TCF3A); anti-FOXA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: FOXA1/1516
• Specificity: The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors.HNF-1 (), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 472

• Applicable Applications for anti-FOXA1 antibody: IHC (Immunohistochemistry)
• Positive Control: HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.
• Immunogen: Recombinant human FOXA1 protein fragment (aa372-472) (exact sequence is proprietary)
• Cellular Localization: Nuclear
• Hu-chromosome Location: 14q21.1
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified FOXA1 Mouse Monoclonal Antibody (FOXA1/1516). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Prostate stained with FOXA1 Mouse Monoclonal Antibody (FOXA1/1516).)

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human Prostate (PIN) stained with FOXA1 Mouse Monoclonal Antibody (FOXA1/1516).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Mouse Monoclonal Antibody (FOXA1/1516).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Prostate stained with FOXA1 Mouse Monoclonal Antibody (FOXA1/1516).)

References

Andre Albergaria, et al. Breast Cancer Research 2009, 11:R40.

NCBI and Uniprot Product Information

• NCBI GI #: 24497501
• NCBI GeneID: 3169
• NCBI Accession #: NP_004487
• NCBI GenBank Nucleotide #: NP_004487.2
• UniProt Accession #: P55317
• Molecular Weight: 49kDa
• NCBI Official Full Name: hepatocyte nuclear factor 3-alpha
• NCBI Official Synonym Full Names: forkhead box A1
• NCBI Official Symbol: FOXA1
• NCBI Official Synonym Symbols: HNF3A; TCF3A
• NCBI Protein Information: hepatocyte nuclear factor 3-alpha
• UniProt Protein Name: Hepatocyte nuclear factor 3-alpha
• UniProt Gene Name: FOXA1
• UniProt Synonym Gene Names: HNF3A; TCF3A; HNF-3-alpha; HNF-3A; TCF-3A
• UniProt Entry Name: FOXA1_HUMAN