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WB (Western Blot)
(Western blot analysis of lysate from 293 cell line using FYN. AAA283521 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Mouse anti-Human FYN Monoclonal Antibody | anti-FYN antibody
FYN Antibody
Gene Names
FYN; SLK; SYN; p59-FYN
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, ELISA
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
FYN, Antibody; FYN Antibody; Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn; FYN; anti-FYN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,kappa
Clone Number
1302CT390.118.237
Specificity
This FYN antibody is generated from a mouse immunized with a recombinant preotein from human FYN.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
537
Applicable Applications for anti-FYN antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Recombinant Protein
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysate from 293 cell line using FYN. AAA283521 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from 293 cell line using FYN. AAA283521 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded R. spleen section using FYN. AAA283521 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded R. spleen section using FYN. AAA283521 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded M. spleen section using FYN. AAA283521 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded M. spleen section using FYN. AAA283521 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. tonsil section using FYN. AAA283521 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. tonsil section using FYN. AAA283521 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using FYN(green) compared to an isotype control of mouse IgG1(blue). AAA283521 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using FYN(green) compared to an isotype control of mouse IgG1(blue). AAA283521 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
Related Product Information for anti-FYN antibody
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta- catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.
Product Categories/Family for anti-FYN antibody
References
Kawakami T.,et al.Mol. Cell. Biol. 6:4195-4201(1986).
Semba K.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:5459-5463(1986).
Rigley K.,et al.J. Immunol. 154:1136-1145(1995).
Goshima N.,et al.Nat. Methods 5:1011-1017(2008).
Mungall A.J.,et al.Nature 425:805-811(2003).
Semba K.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:5459-5463(1986).
Rigley K.,et al.J. Immunol. 154:1136-1145(1995).
Goshima N.,et al.Nat. Methods 5:1011-1017(2008).
Mungall A.J.,et al.Nature 425:805-811(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60762
NCBI Official Full Name
tyrosine-protein kinase Fyn isoform a
NCBI Official Synonym Full Names
FYN proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
FYN
NCBI Official Synonym Symbols
SLK; SYN; p59-FYN
NCBI Protein Information
tyrosine-protein kinase Fyn
UniProt Protein Name
Tyrosine-protein kinase Fyn
UniProt Gene Name
FYN
UniProt Synonym Gene Names
SLK
UniProt Entry Name
FYN_HUMAN
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Product Notes
The FYN fyn (Catalog #AAA283521) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FYN Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FYN can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the FYN fyn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FYN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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