ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with -20 degree C Methanol and using Fyn mouse mAb (dilution 1:50).)
Mouse anti-Human, Mouse Fyn Monoclonal Antibody | anti-FYN antibody
Fyn Antibody
Gene Names
FYN; SLK; SYN; p59-FYN
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Synonyms
Fyn, Antibody; Fyn Antibody; Purified Mouse Monoclonal Antibody (Mab); C syn protooncogene; Fyn; FYN oncogene related to SRC FGR YES; FYN_HUMAN; MGC45350; OKT3 induced calcium influx regulator; P59 FYN; p59-Fyn; Protein tyrosine kinase fyn; Proto oncogene tyrosine protein kinase fyn; Proto-oncogene c-Fyn; Proto-oncogene Syn; Protooncogene Syn; SLK; Src like kinase; Src yes related novel gene; Src-like kinase; Src/yes related novel; Src/yes related novel gene; SYN; Tyrosine kinase p59fyn T; Tyrosine kinase p59fyn(T); Tyrosine-protein kinase Fyn.; anti-FYN antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b
Form/Format
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.09% (W/V) sodium azide and 50% glycerol.
Sequence Length
537
Applicable Applications for anti-FYN antibody
ICC (Immunocytochemistry), WB (Western Blot)
Cellular Location
Cytoplasm.
Nucleus.
Cell membrane.
Note=Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking.
Nucleus.
Cell membrane.
Note=Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking.
Tissue Location
Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages,
especially T- lymphocytes.
Preparation and Storage
Store at -20 °C. Stable for 12 months from date of receipt.
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with -20 degree C Methanol and using Fyn mouse mAb (dilution 1:50).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with -20 degree C Methanol and using Fyn mouse mAb (dilution 1:50).)
WB (Western Blot)
(Western blot detection of Fyn in Hela,3T3,C6,COS7,CEM,Ramos and Jurkat cell lysates using Fyn mouse mAb (1:500 diluted).Predicted band size:59KDa.Observed band size:59KDa.)
WB (Western Blot)
(Western blot detection of Fyn in Hela,3T3,C6,COS7,CEM,Ramos and Jurkat cell lysates using Fyn mouse mAb (1:500 diluted).Predicted band size:59KDa.Observed band size:59KDa.)
Related Product Information for anti-FYN antibody
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta- catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling (By similarity).
Product Categories/Family for anti-FYN antibody
References
Kawakami T.,et al.Mol. Cell. Biol. 6:4195-4201(1986). Semba K.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:5459-5463(1986). Rigley K.,et al.J. Immunol. 154:1136-1145(1995). Goshima N.,et al.Nat. Methods 5:1011-1017(2008). Mungall A.J.,et al.Nature 425:805-811(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59 KDa
NCBI Official Full Name
tyrosine-protein kinase Fyn isoform a
NCBI Official Synonym Full Names
FYN proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
FYN
NCBI Official Synonym Symbols
SLK; SYN; p59-FYN
NCBI Protein Information
tyrosine-protein kinase Fyn
UniProt Protein Name
Tyrosine-protein kinase Fyn
UniProt Gene Name
FYN
UniProt Synonym Gene Names
SLK
UniProt Entry Name
FYN_HUMAN
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Product Notes
The FYN fyn (Catalog #AAA290528) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Fyn Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Fyn can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the FYN fyn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fyn, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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