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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5y cells with GAP43 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit GAP43 Monoclonal Antibody | anti-GAP43 antibody
GAP43 Antibody
Gene Names
GAP43; B-50; PP46
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
GAP43, Antibody; GAP43 Antibody; Axonal membrane protein GAP 43 antibody; Axonal membrane protein GAP-43 antibody; B 50 antibody; Calmodulin binding protein P 57 antibody; F1 antibody; GAP 43 antibody; GAP43 antibody; Growth Associated Protein 43 antibody; Growth-associated protein 43 antibody; Nerve Growth Related Peptide antibody; Nerve growth related peptide GAP43 antibody; NEUM_HUMAN antibody; Neural phosphoprotein B 50 antibody; Neural phosphoprotein B-50 antibody; Neuromodulin antibody; Neuron growth associated protein 43 antibody; PP46 antibody; Protein F1 antibody; QtrA-11580 antibody; QtrA-13071 antibody; anti-GAP43 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SC60-06
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-GAP43 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5y cells with GAP43 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5y cells with GAP43 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining GAP43 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining GAP43 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining GAP43 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining GAP43 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GAP43 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GAP43 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of GAP43 on mouse brain lysates using anti-GAP43 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of GAP43 on mouse brain lysates using anti-GAP43 antibody at 1/1, 000 dilution.)
Related Product Information for anti-GAP43 antibody
GAP-43 (growth associated protein 43, B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1 is a crucial component for regenerative response in the nervous system. It is present at high levels in neuronal growth cones during development and axonal regeneration. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The neuron-specific Elav/Hu family member, HuD, interacts with and stabilizes GAP-43 mRNA in developing neurons and leads to increased levels of GAP-43 protein. Heterozygous GAP-43 knockout mice with GAP-43 levels reduced by one-half dipslay significant memory impairments in cued conditioning or on tests of nociceptive or auditory perception.
Product Categories/Family for anti-GAP43 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,803 Da
NCBI Official Full Name
neuromodulin isoform 1
NCBI Official Synonym Full Names
growth associated protein 43
NCBI Official Symbol
GAP43
NCBI Official Synonym Symbols
B-50; PP46
NCBI Protein Information
neuromodulin; protein F1; neural phosphoprotein B-50; growth-associated protein 43; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neuron growth-associated protein 43
UniProt Protein Name
Neuromodulin
UniProt Gene Name
GAP43
UniProt Entry Name
NEUM_HUMAN
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Product Notes
The GAP43 gap43 (Catalog #AAA312281) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAP43 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GAP43 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GAP43 gap43 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAP43, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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