Standard Curve (Sample)
Mouse Growth Associated Protein 43 (GAP43) ELISA Kit | GAP43 elisa kit
Mouse Growth Associated Protein 43 (GAP43) ELISA Kit
Gene Names
GAP43; B-50; PP46
Reactivity
Mouse
Synonyms
Growth Associated Protein 43 (GAP43); N/A; Mouse Growth Associated Protein 43 (GAP43) ELISA Kit; F1; B-50; PP46; Neuron Growth-Associated Protein 43; Neuromodulin; Axonal Membrane Protein GAP-43; Protein F1; Calmodulin-Binding Protein P-57; Neural Phosphoprotein B-50; GAP43 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of GAP43. No significant cross-reactivity or interference between GAP43 and analogues was observed.
Sequence Length
274
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.054ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GAP43 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GAP43 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GAP43 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GAP43 in mouse tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GAP43. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GAP43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GAP43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GAP43 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GAP43. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GAP43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GAP43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GAP43 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for GAP43 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,766 Da
NCBI Official Full Name
growth associated protein 43
NCBI Official Synonym Full Names
growth associated protein 43
NCBI Official Symbol
GAP43
NCBI Official Synonym Symbols
B-50; PP46
NCBI Protein Information
neuromodulin
UniProt Protein Name
Neuromodulin
UniProt Gene Name
GAP43
UniProt Entry Name
NEUM_HUMAN
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Product Notes
The Mouse GAP43 gap43 (Catalog #AAA20620) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA20620 ELISA Kit recognizes Mouse GAP43. It is sometimes possible for the material contained within the vial of "Growth Associated Protein 43 (GAP43), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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