FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HeLa cells using anti-Glutathione Peroxidase 4/GPX4 antibody (AAA126906).Overlay histogram showing HeLa cells stained with AAA126906 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Glutathione Peroxidase 4/GPX4 Antibody (AAA126906, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Mouse anti-Human Glutathione Peroxidase 4/GPX4 Monoclonal Antibody | anti-GPX4 antibody
Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband (monoclonal, 6I4E7)
Gene Names
GPX4; MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Immunogen affinity purified.
Synonyms
Glutathione Peroxidase 4/GPX4, Antibody; Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband (monoclonal, 6I4E7); anti-GPX4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b
Clone Number
6I4E7
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-GPX4 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HeLa cells using anti-Glutathione Peroxidase 4/GPX4 antibody (AAA126906).Overlay histogram showing HeLa cells stained with AAA126906 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Glutathione Peroxidase 4/GPX4 Antibody (AAA126906, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HeLa cells using anti-Glutathione Peroxidase 4/GPX4 antibody (AAA126906).Overlay histogram showing HeLa cells stained with AAA126906 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Glutathione Peroxidase 4/GPX4 Antibody (AAA126906, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of Glutathione Peroxidase 4/GPX4 using anti-Glutathione Peroxidase 4/GPX4 antibody (AAA126906).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human CACO-2 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified monoclonal antibody (#AAA126906) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Glutathione Peroxidase 4/GPX4 at approximately 20 kDa. The expected band size for Glutathione Peroxidase 4/GPX4 is at 22 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of Glutathione Peroxidase 4/GPX4 using anti-Glutathione Peroxidase 4/GPX4 antibody (AAA126906).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human CACO-2 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified monoclonal antibody (#AAA126906) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Glutathione Peroxidase 4/GPX4 at approximately 20 kDa. The expected band size for Glutathione Peroxidase 4/GPX4 is at 22 kDa.)
Related Product Information for anti-GPX4 antibody
Glutathione peroxidase 4, also known as GPX4, is an enzyme that in humans is encoded by the GPX4 gene. This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-GPX4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 22kDa
Calculated MW: 19kDa/22kDa
Calculated MW: 19kDa/22kDa
NCBI Official Full Name
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
NCBI Official Synonym Full Names
glutathione peroxidase 4
NCBI Official Symbol
GPX4
NCBI Official Synonym Symbols
MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
NCBI Protein Information
phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase
UniProt Protein Name
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
UniProt Gene Name
GPX4
UniProt Synonym Gene Names
PHGPx; GPx-4; GSHPx-4
UniProt Entry Name
GPX4_HUMAN
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Product Notes
The GPX4 gpx4 (Catalog #AAA126906) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband (monoclonal, 6I4E7) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glutathione Peroxidase 4/GPX4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the GPX4 gpx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutathione Peroxidase 4/GPX4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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