IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human melanoma stained with gp100 Rabbit Recombinant Monoclonal Antibody (rPMEL17/6821).)
Mouse gp100/Melanosome/PMEL17/SILV Monoclonal Antibody | anti-SILV antibody
gp100/Melanosome/PMEL17/SILV (Melanoma Marker) Recombinant Mouse Monoclonal Antibody [Clone rPMEL17/6821]
Gene Names
PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
Reactivity
HumanDoes not react with dog and Rat
Applications
Immunohistochemistry
Synonyms
gp100/Melanosome/PMEL17/SILV, Antibody; gp100/Melanosome/PMEL17/SILV (Melanoma Marker) Recombinant Mouse Monoclonal Antibody [Clone rPMEL17/6821]; gp100/Melanosome/PMEL17/SILV (Melanoma Marker); 95kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanosomal matrix protein17, p100, p26, SILV, Silver homolog; anti-SILV antibody
Host
Mouse
Reactivity
Human
Does not react with dog and Rat
Does not react with dog and Rat
Clonality
Monoclonal
Isotype
Mouse/IgG1, kappa
Clone Number
rPMEL17/6821
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-SILV antibody
IHC (Immunohistochemistry)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant full-length human gp100 protein
Cellular Localization
Cytoplasm
Positive Control
COLO-38 or SK-MEL-28 cells. Human melanoma.
Hu-chromosome Location
12q13.2
Unigene
95972
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human melanoma stained with gp100 Rabbit Recombinant Monoclonal Antibody (rPMEL17/6821).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human melanoma stained with gp100 Rabbit Recombinant Monoclonal Antibody (rPMEL17/6821).)
Related Product Information for anti-SILV antibody
The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60 64 kDa), which is subsequently cleaved to generate 26kDa and 34 38kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
70,255 Da
NCBI Official Full Name
Melanocyte protein PMEL
NCBI Official Synonym Full Names
premelanosome protein
NCBI Official Symbol
PMEL
NCBI Official Synonym Symbols
P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
NCBI Protein Information
melanocyte protein PMEL; melanocyte protein mel 17; silver, mouse, homolog of; melanocyte protein Pmel 17; melanosomal matrix protein17; silver locus protein homolog; melanoma-associated ME20 antigen; melanocytes lineage-specific antigen GP100
UniProt Protein Name
Melanocyte protein PMEL
UniProt Gene Name
PMEL
UniProt Synonym Gene Names
D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S
UniProt Entry Name
PMEL_HUMAN
Similar Products
Product Notes
The SILV pmel (Catalog #AAA215920) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The gp100/Melanosome/PMEL17/SILV (Melanoma Marker) Recombinant Mouse Monoclonal Antibody [Clone rPMEL17/6821] reacts with Human Does not react with dog and Rat and may cross-react with other species as described in the data sheet. AAA Biotech's gp100/Melanosome/PMEL17/SILV can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the SILV pmel for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "gp100/Melanosome/PMEL17/SILV, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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